葡萄叶绿体rbcL基因的结构分析  

STRUCTURE OF THE CHLOROPLAST rbc L GENE FROM GRAPE (VITIS VINIFERA

作　　者：黄瑶[1] 马诚[1] 吴乃虎[1] 李朝銮[2] 

机构地区：[1]中国科学院发育生物学研究所 [2]中国科学院成都生物研究所,成都610041

出　　处：《Acta Botanica Sinica》1994年第6期444-451,共8页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：以玫瑰香葡萄（Ｖｉｔｉｓｖｉｎｉｆｅｒａ Ｌ．）为材料，克隆了含有叶绿体ｒｂｃ Ｌ基因的３．１ ｋｂ Ｂａｍ ＨⅠ片段，构建了该基因的限制性酶切图谱，测定了该基因的核苷酸序列。所测的核苷酸序列总长度为２００４ ｂｐ，其中基因的编码区为１４２８ ｂｐ，编码一个含４７５ 个氨基酸的蛋白质，其分子量约为５３ ｋＤ；测定基因的５上游含启动子的部分共３５８ ｂｐ，包括－ １０ 区（ＴＡＡＡＡＴ）、－ ３５区（ＴＴＧＣＧＣ）和ＳＤ 序列（ＧＧＡＧＧ）；基因的３下游区共２１８ ｂｐ，含有３ 个转录茎环终止结构。玫瑰香葡萄ｒｂｃ Ｌ基因编码区的核苷酸序列与烟草、矮牵牛、菠菜、苜蓿、水稻和玉米之间的同源性分别为９１．５％ 、９１．４％ 、９０．２％ 、８９．８％ 、８６．３％ 和８４．５％ ；推导出的氨基酸序列的同源性分别为９２．２％ 、９１．６％ 、９２．２％ 、９３．７％ 、９３．５％ 和９０．１％ 。The 3.1 kb BamH Ⅰ fragment containing the chloroplast rbc L gene from grape (Vitis vinifers L.) had been cloned and the restriction endonuclease map and the nucleotide sequence had been determined.The length of the determined nucleotide sequence was 2004 bp.The coding sequence of the gene was 1428 bp encoding a 475 amino acid polypeptide with a predicted molecular weight of 53 kD.The 5 upstream sequence including the putative promoter was 358 bp, of which the -10 sequence was TAAAAT, the -35 sequence was TTGCGC and the SD sequence was GGAGG.The 3 downstream sequence was 218 bp including three transcription stem loop termination structures.The homologies of this gene with tobacco, petunia, spinach, alfalfa, rice and maize were 91 5%, 91 4%, 90 2%, 89 8%, 86 3% and 84 5%, and the homologies of the putative polypeptide sequences were 92 2%, 91 6%, 92 2%, 93 7%, 93 5% and 90 1%, respectively.

关 键 词：葡萄 叶绿体 结构 RBCL基因 

分 类 号：S663.103.2[农业科学—果树学]