谷子三体系列的建立  

ESTABLISHMENT OF TRISOMIC SERIES OF MILLET (SETARIA ITALICA L. BEAUV.)

作　　者：王润奇[1] 高俊华[1] 王志兴[1] 王志民[1] 

机构地区：[1]河北省农林科学院谷子研究所,石家庄050031

出　　处：《Acta Botanica Sinica》1994年第9期690-695,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：河北省自然科学基金

摘　　要：以谷子（Ｓｅｔａｒｉａ ｉｔａｌｉｃａ （Ｌ．） Ｂｅａｕｖ．）四倍体为母本、二倍体为父本杂交。在２１００ 个杂交后代中得到５ 株三倍体。对三倍体植株授以二倍体花粉，将收获的种子播种后，从所培育的植株中获得９ 种三体共２８３ 株。其中８ 种具有明显的形态特征。The young ears of “Yugu No.1,”a millet (Setaria italica (L.) Beauv.) variety of good quality, high yielding and stress resistance, were chosen to induce callus on N 6 medium. After 3—5 times of subcultures, the callus was treated with colchicine (0 02%) for 48 hours and then transferred back to subculture medium to restore growth, after which the callus was transferred to differentiation medium and subsequently, tetraploid plants were obtained. Through crossing, using tetraploid plants as female parent and diploid plant as male parent, 5 triploid plants were found among 2100 offspring plants. The triploid plants were fertilized with pollens from diploid plants. Among the plants developed from the seeds harvested on triploid plants, 9 kinds of trisomics (totally 283 plants) were identified according to chromosomal number and morphologic feature.Each kind of the trisomics has specific marker feature: (Figures in parentheses represent the number of plants obtained) Triplo 1 (52) has rolling leaves, Triplo 2 (18) is dark green, Triplo 3 (43) is bushy and has degenerated spikelets at the tip of panicles; Triplo 4 (58) has long bristle; Triplo 5 (16) has slender stalks; Triplo 6 (36) has twisted panicle neck; Triplo 7 (44) is semi erect; Triplo 8 (8) has thick panicles; Triplo 9 (8) is pseudonormal (Similar with diploid of “Yugu No. 1”).

关 键 词：谷子 三倍体 三体系列 培育 杂交 

分 类 号：S515.035.1[农业科学—作物学]