双价外壳蛋白基因植物表达载体的构建及马铃薯转基因植株的鉴定  

CONSTRUCTION OF PLANT EXPRESSION VECTORS AND IDENTIFICATION OF TRANSGENIC POTATO PLANTS

作　　者：宋艳茹[1] 李枞[1] 侯林林[1] 张莉枝 马庆虎[1] 彭学贤[2] 崔晓江 周雪荣[2] 王海云[2] 莽克强[2] 

机构地区：[1]中国科学院植物研究所,北京100044 [2]中国科学院微生物研究所,北京100080

出　　处：《Acta Botanica Sinica》1994年第11期842-848,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：中国科学院"八五"攻关重大项目

摘　　要：在克隆了马铃薯Ｘ病毒（ＰＶＸ）、马铃薯Ｙ 病毒（ＰＶＹ）和马铃薯卷叶病毒（ＰＬＲＶ）的外壳蛋白基因的基础上，构建同时包含ＰＶＸ和ＰＶＹ 与ＰＶＹ 和ＰＬＲＶ 两个外壳蛋白基因植物表达框架的表达载体，通过农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ ｔｕｍｅｆａｃｉｅｎｓ）介导转化烟草（Ｎｉｃｏｔｉａｎａｔａｂａｃｕｍ ）和生产上常用的几个马铃薯（Ｓｏｌａｎｕｍ ｔｕｂｅｒｏｓｕｍ ）优良品种：“Ｆａｖｏｒｉｔａ”、“虎头”、“克４”。经ＰＣＲ检测证明外源基因已整合到植物的染色体上，得到批量转基因植株。在转ＰＶＸ＋ＰＶＹ 外壳蛋白基因的烟草上接种ＰＶＸ （５ μｇ／ｍ Ｌ）、ＰＶＹ（２０ μｇ／ｍ Ｌ）病毒。Potato virus X (PVX), potato virus Y (PVY) and potato leafroll virus (PLRV) infection in potato may result in the loss of centrification of seed potatoes and affect the quality and yield of potatoes in agricultural production.The authors cloned coat protein (cp) genes of PVX, PVY and PLRV and constructed two kinds of plant expression vector which contain PVX and PVY or PVY and PLRV cp genes.Three major commercial cultivars of potato and one cultivar of tobacco were transformed via Agrobacterium tumefaciens mediated procedure.Transgenic plants were confirmed by PCR analysis.Transgenic tobacco plants containing both PVX and PVY cp genes were significantly resistant to PVX and PVY infection via mechanical inoculation.

关 键 词：外壳蛋白基因 转基因植株 马铃薯 

分 类 号：S532.032[农业科学—作物学] S188