L-半胱氨酸在玻碳汞膜电极上的直接和间接测定  

Direct and Indirect Determination of L-Cysteine at Glassy Carbon Mercury Film Electrode

作　　者：孙汉文[1] 连靠奇[1] 梁淑轩[1] 刘占峰[1] 李朝昉[1] 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2005年第1期33-37,共5页Journal of Hebei University(Natural Science Edition)

摘　　要：研究了玻碳汞膜电极上L-半胱氨酸(L-Cys)的直接和间接测定方法,并建立了间接和直接测定的最佳条件.在pH=4.56的缓冲溶液中L-Cys于-0.44V处有一灵敏的不可逆的循环扫描还原峰,其峰电流与L-Cys的浓度在1×10-6～1×10-4mol/L内呈良好线性关系(r=0.9989).另外,根据L-Cys在适当的pH条件下与铜离子的络合作用,通过阳极溶出伏安法测定Cu2+来间接测定L-Cys的含量,本法L-Cys浓度在2×10-7～3×10-6mol/L内呈良好线性关系(r=0.9992).2种方法可成功地用于合成样品中L-Cys的测定.The direct and indirect determination of L_Cysteine on glassy carbon mercury film electrode has been studied, and the optimized conditions for the determination of L_Cysteine has been founded. In the buffer solution of pH=4.56,a sensitive reductive peek of L_Cysteine was observed at-0.44 V on the mercury film electrode. Linear relationship was obtained between the peek current (I_P) and the concentration of L_Cysteine in the range from 1×10^(-6) to 1×10^(-4) mol/L. Further more, based on the complex formation between copper ion and LCysteine, the amount of LCysteine can be indirectly determined by measuring the amount of Cu^(2+) in the solution with anodic stripping voltammetry. The linear range was 2×10^(-7)～3×10^(-6)mol/L. The methods have been successfully applied to the determination of LCysteine in synthetic sample.

关 键 词：L-半胱氨酸 玻碳汞膜电极 循环伏安法 阳极溶出伏安法 

分 类 号：O657.13[理学—分析化学]