胎盘血管生长因子在妊娠高血压综合征的表达及意义  

Expression of placental vascular growth factor in pregnancy-induced hypertension

作　　者：胡志英[1] 方马荣[2] 王伊萍[1] 陈丽[1] 吴林珍[1] 李继承[2] 

机构地区：[1]浙江省中西医结合医院妇产科,杭州310003 [2]浙江大学医学院

出　　处：《中华围产医学杂志》2005年第2期73-76,i001,共5页Chinese Journal of Perinatal Medicine

基　　金：浙江省杭州市卫生局资助课题(基金编号99A17)

摘　　要：目的　通过比较正常孕妇与妊娠高血压综合征(妊高征)患者胎盘分泌的血管生长因子,包括血管内皮生长因子、碱性成纤维细胞生长因子及血小板来源生长因子的表达变化,探讨血管生长因子在妊高征的表达及意义。　方法　采集正常孕妇(15例)及妊高征患者(19例)的胎盘组织,采用免疫组织化学PAP法结合计算机显微图像分析软件,检测胎盘血管生长因子的表达。　结果　妊高征组中,中、重度妊高征患者胎盘的血管内皮生长因子阳性反应平均灰度值为 61.6±3.7,明显低于正常妊娠组的71.1±5.8和轻度妊高征的70.9±8.9,差异有统计学意义(P<0.01);碱性成纤维细胞生长因子阳性反应平均灰度值为 60.9±8.8,较正常妊娠组的 79.7±7.6 和轻度妊高征的78.4±10.4明显下降,差异有统计学意义(P<0.01);血小板来源生长因子阳性反应平均灰度值为37.5±5.6,较正常妊娠组的22.1±3.1和轻度妊高征的 21.8±4.3 明显增加,差异亦有统计学意义(P<0.01)。　结论　胎盘血管生长因子表达异常,可能导致胎盘血管面积下降,胎盘血管壁张力增高、痉挛,从而影响胎盘的血液供应,导致妊高征的一系列病理生理改变;表达异常的程度与妊高征病情轻重有关。Objective To investigate the expression of vascular growth factors including vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) and platelet-derived growth factor (p-DGF) in placentae of pregnancy-induced hypertension(PIH) patients and their significance. Methods Placental tissues of 15 normal gravidas and 19 PIH women were collected. The expression of vascular growth factors were detected by immunohistochemistry (PAP) combined with the analysis of computer microphotography. Results The average grey value difference (GVD) of VEGF was 61.6±3.7 in moderate and severe PIH group, which was significantly lower than that of the normal control group (71.1±5.8) and mild PIH (70.9±8.9, P<0.01); the average GVD of bFGF in the moderate and severe PIH group (60.9±8.8) was significantly lower than that of the control group (79.7±7.6) and mild PIH group (78.4±10.4, P<0.01) and the average GVD of p-DGF in the moderate and severe PIH group (37.5±5.6) was higher than that in the control group (22.1±(3.1)) and mild PIH group(21.8±4.3, P<0.01). Conclusions Abnormal expression of the placental vascular growth factors might result in decreased placental blood vessel distribution, increased tension or spasm of blood vessel and lead to the pathologic and physiologic changes in PIH.

关 键 词：血管生长因子 表达及 碱性成纤维细胞生长因子 妊娠高血压综合征(妊高征) 血管内皮生长因子 免疫组织化学PAP法 平均灰度值 轻度妊高征 计算机显微图像 源生长因子 妊高征患者 阳性反应 正常妊娠 病理生理改变 正常孕妇 

分 类 号：R714.246[医药卫生—妇产科学]