肺炎支原体膜脂蛋白通过TLR2介导mICAM-1表达的上调  

The lipid associated membrane protein of Mycoplasma pneumoniae induces the upregulation of mICAM-1 on THP-1 cells via TLR2 pathway

作　　者：董碧麟[1] 汤纪路[1] 白瑞珍[1] 宗润芝[1] 刘先洲[1] 

机构地区：[1]武汉大学医学院微生物教研室,430071

出　　处：《中华微生物学和免疫学杂志》2005年第2期103-106,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　观察肺炎支原体膜脂蛋白 (Mp LAMP)对THP 1 (前单核白血病细胞系 )细胞TLR2 (Toll样受体 )与mICAM 1 (膜结合型细胞间黏附分子 1 )表达的影响 ,探讨膜脂蛋白中的活性成分及二者表达的相互关系。方法　用流式细胞术检测Mp LAMP刺激组、抗人TLR2功能纯化抗体(TL2 .1 )阻断组及消化酶处理组的THP 1细胞mICAM 1和TLR2的表达水平。结果　与对照组相比 ,不同刺激浓度的Mp LAMP对THP 1细胞膜上TLR2及mICAM 1的表达均有明显的上调作用 (P <0 .0 5 ) ,且表达水平的增加对Mp LAMP的刺激具有浓度依耐性 ;用TL2 .1将THP 1细胞膜上的TLR2阻断后 ,Mp LAMP对mICAM 1表达的诱导作用明显受到抑制 (P <0 .0 1 ) ,其阻断效果随TL2 .1量的增加而加强 ;酶消化试验证实Mp LAMP的活性成分是其脂质部分。相关分析表明 ,mICAM 1在THP 1细胞上的表达水平与TLR2密切相关 (r=0 .989,P <0 .0 1 )。结论　肺炎支原体膜脂蛋白通过TLR2介导上调THP 1细胞mICAM 1的表达 ,在一定程度上影响着肺炎支原体感染所导致的炎性反应的强弱。Objective To observe the effect of lipid associated membrane protein of Mycoplasma pneumoniae (Mp-LAMP) on the expression of mICAM-1 and TLR2 on the THP-1 cells and further study the target component of the membrane lipoprotein as well as the relationship of their expression. Methods The flow cytometry assay was adopted to analyze the expression of mICAM-1 and TLR2 on the THP-1 cells, which were respectively treated with only Mp-LAMP, Mp-LAMP blocked by the purified anti-human TLR2 monoclonal antibody (TL2.1) , Mp-LAMP digested with different enzymes. The results were compared to that of untreated cells. Results All the Mp-LAMP of different densities had the significant inducing ability to upregulate the expression of mICAM-1 and TLR2 on the THP-1 cells as compared to those of untreated cells(P<0.05) and the increment of their expression levels was dependent on the density of Mp-LAMP. When the TLR2 on the THP-1 cells was blocked by the TL2.1, the inducing ability of Mp-LAMP at 25?μg/ml to upregulate the expression of mICAM-1 and TLR2 on THP-1 cells was severely inhibited(P<0.01) and the extent of inhibition depended on the dose of TL2.1. The results of enzyme digestion showed the component with inducing ability was the lipid moiety of Mp-LAMP. The correlation analysis found that there existed a close relationship between the upregulation of the expression of mICAM-1 and that of TLR2 on the THP-1 cells treated by Mp-LAMP(r2?0.989, P<0.01). Conclusion Mp-LAMP upregulates the expression of mICAM-1 on the THP-1 cells via the TLR2 pathway and, to a certain extent, have an effect on the intensity of inflammation triggered by Mycoplasma pneumoniae.

关 键 词：ICAM-1表达 TLR2 THP-1细胞 介导 白通 体膜 细胞问黏附分子 Toll样受体 流式细胞术检测 肺炎支原体感染 白血病细胞系 膜脂蛋白 活性成分 膜结合型 相互关系 上调作用 诱导作用 阻断效果 相关分析 炎性反应 水平 酶处理 

分 类 号：R392[医药卫生—免疫学]