CXCL16抗体可阻断BCG和LPS诱导的小鼠免疫性肝损伤  

Protective role of CXCL16 antibody in murine immunological liver injury induced by BCG and LPS

作　　者：徐焕宾[1] 龚燕萍[1] 储以微[1] 蒋正刚[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫学系,教育部分子医学重点实验室,上海200032

出　　处：《中华微生物学和免疫学杂志》2005年第2期111-115,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家教育部科学技术重点项目 (0 3 0 63 ) ;国家杰出青年科学基金 (3 992 5 0 3 1) ;国家重点基础研究发展计划资助项目(2 0 0 1CB5 10 0 0 6)

摘　　要：目的　研究CXCL1 6抗体体内阻断对小鼠免疫性肝损伤的影响。方法　克隆、表达和纯化小鼠趋化因子CXCL1 6活性蛋白 ,免疫动物制备特异性抗体。抗体体内注射免疫性肝损伤模型小鼠 ,观察CXCL1 6抗体对肝脏损伤、ALT水平及小鼠生存率的影响。结果　获得了高纯度的活性CXCL1 6重组蛋白及其特异性抗体 ,特异性抗体阻断明显改善肝脏的病理损伤 ,降低血清中ALT水平、延长模型组小鼠的存活时间。结论　获得了具有趋化活性的CXCL1 6蛋白和特异性抗体 ,CXCL1 6抗体对卡介苗和脂多糖诱导的小鼠肝损伤具有显著保护作用。Objective To investigate the effects of CXCL16 antibody in murine immunological liver injury. Methods Mouse CXCL16 chemokine was expressed and purified with prokaryotic expression systems. The chemotactic activity was analyzed by chemotaxis assay. CXCL16 antibody was prepared and purified. The specificity and neutralization of specific antibody were further detected with double immune diffuse. In immunological liver injury model induced by BCG and LPS, the neutralizing CXCL16 antibody was administrated in vivo, the hepatic necrosis, serum ALT level and survive rate were evaluated. Results Administration of CXCL16 antibody protected the mice from liver injury with a significant reduction of both ALT level and hepatic lesion. The life of animal was prolonged by CXCL16 treatment. Conclusion CXCL16 antibody has a potential protective role in murine liver injury induced by BCG and LPS.

关 键 词：CXCL16 免疫性肝损伤 LPS诱导 阻断 BCG 特异性抗体 ALT水平 小鼠肝损伤 脂多糖诱导 活性蛋白 趋化因子 免疫动物 模型小鼠 体内注射 肝脏损伤 病理损伤 存活时间 趋化活性 保护作用 生存率 重组蛋 高纯度 低血清 卡介苗 

分 类 号：R392[医药卫生—免疫学]