GM-CSF对抗原化抗体基因免疫T_H细胞应答的调节作用  

Effect of GM-CSF on the T helper cell response induced by gene immunization with antigenized antibody

作　　者：夏明灿[1] 储以微[1] 林怡[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫学系,上海200032

出　　处：《中华微生物学和免疫学杂志》2005年第2期116-120,共5页Chinese Journal of Microbiology and Immunology

基　　金：上海 -联合利华研究与发展基金资助课题 (2 0 10 ) ;国家自然科学基金面上项目 (3 0 170 867) ;国家"863"计划项目(2 0 0 4AA2 15 2 42 )

摘　　要：目的　研究GM CSF(粒 单核巨噬细胞集落刺激因子 )与抗原化抗体联合基因免疫时 ,GM CSF对TH 细胞应答的调节作用。方法　在编码免疫球蛋白重链的质粒中分别克隆黏蛋白 1(MUC1 )中特异性PDTRP抗原表位和GM CSF编码基因 ,构建含PDTRP和GM CSF编码基因的抗原化抗体表达重组体。经脾免疫和肌肉加强的方式免疫BALB c小鼠。采用ELISA方法检测小鼠血清中特异性抗体的水平及其亚类并动态观察 ;RT PCR方法检测TH 细胞分化中细胞因子和转录因子的表达水平。结果　抗原化抗体基因免疫能诱导机体产生免疫应答。GM CSF增强抗原化抗体基因免疫诱生的抗PDTRP特异性IgG抗体水平并且伴随IgG1 IgG2a显著性升高 ,同时可增强淋巴细胞TH2型细胞因子及转录因子 (IL 4、GATA 3)mRNA的表达水平。结论　GM CSF在增强抗原化抗体基因免疫诱导的免疫应答的同时使得免疫应答向TH2方向偏移。Objective To investigate the effect of GM-CSF on the T helper cell response induced by gene immunization combined with GM-CSF and antigenized antibody. Methods A DNA plasmid that contains epitope of mucin 1 polypeptide PDTRP and immune adjuvant GM-CSF was constructed with antigenized antibody. A specific immune response against PDTRP was elicited via intrasplenic gene immunization and boosted via i.m gene immunization. The sera were collected and the levels of specific antibody were detected by ELISA. The expressions of key molecules in T H cell differentiation were determined by RT-PCR. Result The specific immune response was induced by antigenized antibody. GM-CSF enhanced the levels of IgG with IgG2a/IgG1 ratio increasing significantly and the expression of T H2-type cytokines and transcription factors. Conclusion GM-CSF could enhance the immune response and improve T H2 type immune response induced by gene immunization with antigenized antibody.

关 键 词：GM-CSF 抗原化抗体 调节作用 细胞应答 单核巨噬细胞集落刺激因子 BALB/c小鼠 RT-PCR方法 TH2型细胞因子 免疫球蛋白重链 ELISA方法 IGG抗体水平 联合基因免疫 TH细胞分化 免疫应答 编码基因 转录因子 特异性抗体 IGG2A 

分 类 号：R392[医药卫生—免疫学]