SARS-CoV核衣壳蛋白用于SARS血清学诊断的研究  

Nucleocapsid protein of SARS coronavirus as a marker for the SARS serodiagnosis

作　　者：王压娣 郝卫[1] 廖志勇[1] 梅亚波[1] 尹锴 车小燕[1] 

机构地区：[1]第一军医大学珠江医院中心实验室,广州510282

出　　处：《中华微生物学和免疫学杂志》2005年第2期152-155,共4页Chinese Journal of Microbiology and Immunology

基　　金：科技部防治非典型肺炎科技攻关项目 ;广东省防治非典型肺炎科技攻关项目 (2 0 0 3 80 )

摘　　要：目的　研究SARS患者血清中抗SARS冠状病毒 (SARS CoV)核衣壳 (N)蛋白特异性抗体的产生规律 ,评价N蛋白在SARS诊断中的作用。方法　采用间接ELISA法测定 2 5 0例临床确诊、1 88例临床疑似、6 2例临床排除SARS患者血清中针对N蛋白IgG抗体 ,并与其SARS CoVIgG总抗体水平进行比较。结果　临床确诊病例针对N蛋白和全病毒特异性抗体IgG的阳性率均随着病程的延长而增高。N IgG在发病第 1周检出阳性率为 3.4 5 %(4 1 1 6 ) ,第 2周为 6 1 .76 %(42 6 8) ,第 3周为81 .82 %(1 8 2 2 ) ,2 2～ 93d达到 1 0 0 %(44 4 4) ;SARS CoVIgG第 1周检出阳性率为 4 .31 %(5 1 1 6 ) ,第 2周为 5 5 .88%(38 6 8) ,第 3周为 77.2 7%(1 7 2 2 ) ,2 2～ 93d达到 1 0 0 %(44 4 4) ;临床疑似病例N IgG检出阳性率为 3.1 9%(6 1 88) ,SARS CoVIgG检出阳性率为 2 .6 6 %(5 1 88) ;临床排除病例N IgG和SARS CoVIgG检出阳性率均为 6 .4 5 %(4 6 2 )。两种方法检测阳性率差异无统计学意义 (χ2 =0 .1 80 ,P <0 .0 0 1 ) ;两者之间符合率为 98.2 0 %(491 5 0 0 ) ,正常人群SARS CoVN IgG阳性率为 1 .88%(1 4 74 5 )。结论　SARS CoVN重组蛋白对于SARS诊断试剂的研究具有重要价值。Objective To study the temporal profile of the specific antibody against nucleocapsid(N) protein in patients with severe acute respiratory syndrome (SARS) and to evaluate the N protein used as diagnostic marker of SARS serodiagnosis. Methods A tolal of 500 sera, including 250 clinical documented, 188 clinical suspected and 62 clinically excluded SARS patients were analyzed by both the recombinant N protein and lysates of SARS-CoV based indirect enzyme-linked immunosorbent assay (ELISA). Results Among 250 sera from clinical documented patients with SARS, IgG responses to the recombinant N protein was detected in 4 of 116 (3.45%) at first week, 42 of 68 (61.76%) at second week , 18 of 22( 81.82% ) at third week and 44 of 44 (100%) from day 22 to 93 after the onset of symptoms respectively. In comparison, the positive rates of the IgG against SARS-CoV were 4.31% (5/116), 55.88% (38/68), 77.27% (17/22) and 100% (44/44) in the same four phases respectively. In 188 specimens from clinical suspected SARS patients, 3.19%(6/188) was IgG positive for N and 2.66% (5/188) for SARS-CoV respectively. Both IgG positive rate of the N and of SARS-CoV was 6.45% (4/62) in the specimens from clinical excluded SARS patients. The McNemar t test showed that the difference was not significant between the results of two methods for SARS serodiagnosis (χ2=0.180, P<0.001). The result of the indirect ELISA based N-protein showed a 98.20% (491/500) agreement with that of the ELISA assay with SARS-CoV. Furthermore, a few samples of sera (1.88%, 14/745) from health donors may be cross-reacted with the recombinant N protein. Conclusion The recombinant N protein of SARS-CoV may be a cadidate of diagnostic marker for SARS serodiagnosis.

关 键 词：血清学诊断 核衣壳蛋白 冠状病毒(SARS-CoV) 间接ELISA法 SARS诊断试剂 SARS患者 阳性率 特异性抗体 抗体IGG 病毒特异性 N蛋白 产生规律 抗体水平 确诊病例 疑似病例 正常人群 重组蛋白 临床 检出 统计学 符合率 

分 类 号：R446.61[医药卫生—诊断学]