生长激素mRNA在蓝太阳鱼垂体外组织中的表达分布  

The Profile of Growth Hormone Gene Expression in Extrapituitary Tissues of Lepomis cyanellus

作　　者：曹运长[1] 文红波[1] 李文笙[2] 林浩然[2] 

机构地区：[1]南华大学生命科学与技术学院,湖南衡阳421001 [2]中山大学水生经济动物研究所,广东省水生经济动物繁殖重点实验室,广东广州510275

出　　处：《Zoological Research》2005年第2期174-178,共5页动物学研究（英文）

基　　金：国家自然科学基金倾斜专项资助项目(39970586);广东省自然科学基金资助项目(20023002);广东省科技计划项目(2002B21508)

摘　　要：应用半定量RT PCR方法和Southern杂交技术,系统地研究了生长激素(GH)基因在蓝太阳鱼垂体外组织中的表达情况。在建立检测蓝太阳鱼GHmRNA表达的半定量RT PCR扩增条件之后,分别对蓝太阳鱼雄性幼鱼(6月龄)和雄性成鱼(1年龄)的12个组织部位中GHmRNA的表达进行了检测。结果表明,除了在垂体之外,还在肌肉、性腺、鳃、心脏、脑、肾脏6个组织检测到GHmRNA的表达,但各组织间的表达水平存在差异,而在脾脏、肝脏、胃3个组织未检测到表达;肌肉组织中的表达水平从幼鱼到成鱼后明显提高,同时观察到在幼鱼和成鱼的性腺组织中存在着较高水平的表达。本研究表明,GH基因在蓝太阳鱼的垂体外组织中存在着广泛的表达,由此提示,蓝太阳鱼GH可能以旁分泌或自分泌的方式对其生长繁殖起着重要的作用。By semi-quantitative RT-PCR method and Southern blotting technology, growth hormone (GH) gene expression was determined in extra-pituitary tissues of green sunfish, Lepomis cyanellus. After the condition of semi-quantitative RT-PCR was established, 12 tissues of 6 months old (larvae stage) and 1 year old (mature stage) male L. cyanellus were detected. The results showed that GH gene was expressed in muscle, gonad, gill, heart, brain, and kidney in different level besides pituitary gland, and that no GH expression was detected in spleen, liver and stomach. Unexpectedly and interestingly, GH expression in muscle of adult fish was distinctly higher than that of sexually immature fish. In addition, the transcript levels of GH mRNA in the gonad of larvae and adult fishes were high. The data presented here provide the first systematic report of universal GH gene expression in extra-pituitary tissues in fish, suggesting that GH through autocrine or paracrine pathway exerted its possible important local physiological function during development and growth of L. cyanellus.

关 键 词：蓝太阳鱼 生长激素 半定量RT-PCR 基因表达 MRNA 垂体 

分 类 号：Q57[生物学—生物化学]