大鼠眼球和泪腺中睫状神经营养因子及其受体的组织定位  

LOCALIZATION OF CILIARY NEUROTROPHIC FACTOR AND ITS RECEPTOR IN EYE BALL AND LACRIMAL GLAND OF RATS

作　　者：许家军[1] 刘芳[1] 张志英[1] 吴云霞[2] 路长林[3] 

机构地区：[1]第二军医大学解剖学教研室神经生物学教研室,上海200433 [2]福建医科大学解剖学教研室 [3]第二军医大学神经生物学教研室,上海200433

出　　处：《中国组织化学与细胞化学杂志》2005年第2期173-176,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：全军医药卫生科研基金重点项目 (01Z065)

摘　　要：目的　观察睫状神经营养因子(CNTF) 及其受体CNTFRα在大鼠眼球、泪腺上的分布情况。方法　取雄性SD大鼠两侧眼球和泪腺, 作石蜡切片, 用免疫组织化学ABC法染色检测眼球、泪腺中CNTF和CNTFRα的免疫阳性反应。结果　CNTF和CNTFRα的免疫阳性反应产物在眼球和泪腺的组织定位基本相同, 包括角膜上皮细胞、固有层神经纤维、角膜细胞、内皮细胞, 虹膜上皮细胞, 睫状体上皮细胞, 晶状体上皮, 视网膜色素上皮细胞、苗勒细胞、节细胞层细胞, 球结膜上皮细胞, 泪腺腺细胞及导管上皮。结论　CNTF及其受体选择性地分布于眼球和泪腺的一些细胞中, 且多数细胞与房水和泪液的产生和接触有关。Objective To observe the localization of ciliary neurotrophic factor (CNTF) and its receptorα(CNTFRα) in the eyeball and lacrimal gland of rats. Methods ABC immunohistochemistry was used to detect the immunoreactivity of CNTF and CNTFRα in the eyeball and lacrimal gland of SD male rats.Results The immunohistochemical localization of CNTF was similar to that of CNTFRα in the eyeballand lacrimal gland of rats. The cells expressing CNTF and CNTFR included epithelium, nerve fiber, stroma cell and endothelium of cornea, epithelium of iris, ciliary body, lens, pigment epithelium, Müller cell and cells in the ganglion cell layer of retina, epithelium of conjunctiva, gland cell and duct epithelium of lacrimal gland. Conclusion CNTF and CNTFRα are specifically distributed in some cells of the eyeball and lacrimal gland, which are often related to producing and contacting aqueous humor and lacrimal liquid.

关 键 词：睫状神经营养因子 睫状神经营养因子受体α 眼球 泪腺 大鼠 

分 类 号：R322.91[医药卫生—人体解剖和组织胚胎学] R338.14[医药卫生—基础医学]