口腔念珠菌的PCR ITS1-ITS2和RAPD基因分型  

Oral Candida Genotyping by PCR ITS1-ITS2 and RAPD

作　　者：赵民[1] 周曾同[1] 张济[2] 唐国瑶[1] 吴岚[1] 沈雪敏[1] 

机构地区：[1]上海第二医科大学口腔医学院第九人民医院口腔内科,上海200011 [2]中国科学院上海生命科学院上海第二医科大学健康科学研究所

出　　处：《上海第二医科大学学报》2005年第4期342-344,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市卫生局科技发展基金(044005)资助项目.

摘　　要：目的探讨对口腔念珠菌准确可靠的基因分型方法。方法9株临床菌株经YBCTestKit鉴定为7株白色念珠菌(简称白念),1株热带念珠菌,1株光滑念珠菌。分别采用PCRITS1ITS2和RAPD两种基因分型方法对其进行基因分型,并比较其结果。结果PCRITS1ITS2方法将7株白念分为2型,将2株非白念准确鉴定为热带念珠菌和光滑念珠菌;用RAPD方法将7株白念分为6型,推测另外2株为非白念。结论两种分型方法对白念种内的分型结果不完全一致。PCRITS1ITS2分型法能准确地区分非白念。Objective To genotype 9 candida Clinical isolates by PCR ITS1-ITS2 and RAPD methods, and compare the results of these two methods. Methods Nine Candida species identified by YBC Test Kit included 7 C.albicans,1 C.tropicalias and 1 C.glabrates. PCR ITS1-ITS2 and RAPD methods were used to genotype 9 Candida species which were isolated from the oral cavity. Results Seven C.albicans were divided into 2 genotypes by PCR ITS1-ITS2,and 2 Candida clinical isolates were identified as 1 C.tropicalias and 1 C.glabrates. Seven C.albicans were divided into 6 genotypes by RAPD,two isolates speculated as non- C.albicans. Conclusion The results of the genotypes between the two methods were not consistent completely. The results suggest that the PCR ITS1-ITS2 method is useful to genotype candida.

关 键 词：白色念珠菌 口腔 基因分型 念珠菌感染 

分 类 号：R446.5[医药卫生—诊断学]