整合素α_1在增生性瘢痕组织中表达的定量研究  

Quantitative Study of α_1 Integrin Expression in Hypertrophic Scar Tissues

作　　者：马进[1] 范志宏[1] 

机构地区：[1]上海第二医科大学仁济医院整形外科,上海200127

出　　处：《上海第二医科大学学报》2005年第4期371-373,377,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金(30000176)资助项目.

摘　　要：目的研究增生性瘢痕组织内整合素α1表达水平及其意义。方法应用荧光定量PCR和巢式PCR技术检测10例增生性瘢痕组织中整合素α1的表达,并以10例正常皮肤组织作为对照。结果样品与内参照物基因组拷贝数的比值(α1Integrin/GAPDH×100%),增生性瘢痕组为(394.96±27.05)%,正常皮肤组为(20.40±4.88)%,两组差异显著(P<0.01)。结论应用荧光定量和巢式PCR方法可对细胞内整合素进行较准确的测定,方法简便且敏感。增生性瘢痕组织中整合素α1的高表达可能是瘢痕增生的重要因素。Objective To investigate the expression of α 1 integrin in hypertrophic scar tissues and discuss its function in scar formation. Methods The expressive levels of α1 integrin in specimens were determined by FQ-PCR technique and nest PCR. Results The expressive levels of α1 integrin in hypertrophic scar tissues are higher than those in normal skins. Conclusion FQ-PCR technique and nest PCR could be applied in exploring the level of integrin,and the method is simple, sensitive and accurate. The higher expressive levels of α1 integrin in hypertrophic scar tissues may contribute to the formation of scar.

关 键 词：增生性瘢痕 整合素 荧光定量PCR 巢式PCR 

分 类 号：R622[医药卫生—整形外科]