纳米级羟基磷灰石梯度涂层对成骨细胞表型因子表达的影响  

Effect of Nanophase Hydroxyapatite Biograde-Coated Material on Osteoblastic Phenotype Expression

作　　者：谭延斌[1] 王毅[1] 杨庆铭[1] 邓廉夫[1] 齐进[1] 朱雅萍[1] 曾绍先[2] 

机构地区：[1]上海第二医科大学瑞金医院骨科上海市伤骨科研究所,上海200025 [2]中国科学院上海硅酸盐研究所

出　　处：《上海第二医科大学学报》2005年第4期382-384,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市纳米专项基金(0144nm064)资助项目.

摘　　要：目的研究纳米级羟基磷灰石梯度涂层对成骨细胞表型因子表达的影响。方法对生长于纳米级羟基磷灰石梯度涂层上成骨细胞表达的I型前胶原羧基端肽(PICP)、碱性磷酸酶(ALP)和骨谷氨酸蛋白(BGP),于体外培养第1、5、9、13d时进行定量分析,扫描电镜观察细胞形态学改变。以普通级羟基磷灰石涂层和钛合金圆片作为对照。结果纳米级羟基磷灰石梯度涂层组的PICP、ALP和BGP表达均高于对照组,成骨细胞形态好于对照组。结论纳米级羟基磷灰石梯度涂层材料能促进成骨细胞表型因子的表达。Objective To study the effect of nanophase hydroxyapatite biograde-coated plates on osteoblastic phenotype. Methods Osteoblasts were cultured on nanophase hydroxyapatite biograde-coated plates, hydroxyapatite biograde-coated plates and Ti-6Al-4V hydroxyapatite biograde-coated plates, the expressions of carboxyterminal propeptide of type I procollagen (PICP), alkaline phosphatase (ALP) and, bone gla protein (BGP) were assayed at 1, 5, 9 and 13 d. The plates were observed with SEM at the same time. Results The expressions of PICP, ALP and BGP of the cells cultured on nanophase hydroxyapatite material were higher than those of the control group, and the cellular shape was better. Conclusion The expression of osteoblastic phenotype factors was stimulated by nanophase hydroxyapatite biograde-coated material.

关 键 词：纳米级 羟基磷灰石 成骨细胞 表型 I型前胶原羧基端肽 碱性磷酸酶 

分 类 号：R318.08[医药卫生—生物医学工程] R329.26[医药卫生—基础医学]