微柱高效液相色谱法测定环境样品中的铁、钴、镍、铜、锌、锰  

Determination of Iron, Cobalt, Nickel, Copper, Zinc and Manganese in Environment al Samples with Microcolumn High Performance Liquid Chromatography

作　　者：司云森[1] 胡秋芬[2,3] 杨光宇[4] 尹家元[4] 

机构地区：[1]昆明理工大学理学院 [2]玉溪师范学院化学系 [3]云南大学化学系昆明650091 [4]云南大学化学系

出　　处：《化学通报》2005年第4期314-317,共4页Chemistry

摘　　要：研究了用 2-(2-喹啉偶氮 )-间苯二酚 (QAR)为柱前衍生试剂 ,以WatersXterraTM RP18(1 .0×5 0mm ,2. 5 μm)微柱为固定相 ,60 %的甲醇 (内含 0 . 5 %的醋酸 )为流动相 ,高效液相色谱分离、二极管矩阵检测器测定铁、钴、镍、铜、锌和锰的方法。根据信噪比 (S N =3 )得各金属离子的检测限分别为 :铁3 μg L、钴 4μg L、镍 2 μg L、铜 4μg L、锌 5 μg L、锰 8μg L ,方法用于环境样品中痕量铁、钴、镍、铜、锌、锰的测定 ,相对标准偏差在 1 . 6%～ 3 . 5 %之间 ,标准回收率为 93 %～ 1 0 7%。A new method for the determination of ir on, cobalt, nickel, copper, zinc and manganese in environmental samples with mic rocolumn high performance liquid chromatography using 2_(2_quinolinylazo)_ resor cin (QAR) as chelating reagent was described. The iron, cobalt, nickel, copper, zinc and manganese chelates of QAR can be separated on a Waters Xterra TM RP 18 microcolumn (1.0×50mm, 2.5μm) with 60% methanol (containing 0. 5% of acetic acid) as mobile phase and monitored with photodiode array detector. The detection limit (S/N=3) is 3, 4, 2, 4, 5 and 8μg/L for iron, cobalt, n ickel, c opper, zinc and manganese, respectively. This method can be applied to the deter mination of iron, cobalt, nickel, copper, zinc and manganese in environmental sa mples. The relative standard deviations are 1.6%～3.5%. The standard recoveri es are 93%～107%.

关 键 词：微柱高效液相色谱法 环境样品 测定 钴 镍 铜 锌 锰 柱前衍生试剂 液相色谱分离 相对标准偏差 标准回收率 间苯二酚 喹啉偶氮 金属离子 固定相 流动相 检测器 二极管 检测限 信噪比 痕量铁 矩阵 

分 类 号：O657.7[理学—分析化学]