CD34^+HPC来源的树突状细胞体外扩增和功能研究  

Expansion and Function of Dendritic Cells Derived from Human Cord Blood CD34 ^+ Cells

作　　者：吴明媛[1] 黄勇[1] 朱庭 朱一蓓[1] 夏瑜[1] 张学光[1] 

机构地区：[1]苏州大学生物技术研究所,江苏苏州215007 [2]苏州市第二人民医院妇产科,江苏苏州215002

出　　处：《苏州大学学报（医学版）》2005年第1期4-6,共3页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助课题(39870825)江苏省卫生厅资助项目(H200340)

摘　　要：目的研究脐血CD34+HPC体外扩增诱导产生功能性树突状细胞(DCs)的有效方案。方法Ficoll分离脐血获得单个核细胞,免疫磁珠阳性分选CD34+HPC,3H—TdR检测DCs激发异体T细胞增殖的能力,MTT法检测特异性CTL的杀伤能力。结果SCF+FL+GM-CSF+IL-4诱导生成的DCs激发T细胞增殖和特异性CTL的杀伤能力均高于其他组。结论SCF+FL+GM-CSF+IL-4可有效扩增CD34+HPC并诱导产生功能性DCs。Objective To probe the effective program of expansion and function of dendritic cells (DCs) derived from human cord blood CD34+ hematopoietic precursor cells(HPC). Methods The isolation of CD34 + cells from human cord blood was used with mini-MACS. The proliferation of T cells activated by allo-DCs was measured with 3H-TdR incorporation. The cytotoxic activity of special CTL was measured with MTT. Results The proliferation of T cells and the cytotoxic activity of CTL stimulated by DCs inculture with SFG4 were higher than those in other groups. Conclusion CD34 + HPC could expand effectively and be induced into DCs with the combination of SCF, Flt-3 Ligand, GM-CSF and IL-4.

关 键 词：CD34^+HPC 细胞因子 树突状细胞 

分 类 号：R392.12[医药卫生—免疫学]