水分胁迫对不同小麦品种光系统功能基因psbA、psbD及cab表达的影响(英文)  

Effects of Water Stresses on the Expressions of Functional Genes, psbA, psbD and cab, for the Photosystem Ⅱ of Different Wheat Varieties

作　　者：刘文娟[1] 袁澍[1] 张年辉[1] 雷韬[1] 段辉国[2] 梁厚果[1] 杜林方[1] 代其林[1] 林宏辉[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川内江师范学院化学与生命科学系,四川内江641112

出　　处：《西北植物学报》2005年第4期714-718,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目30170557;教育部博士点基金项目;四川省科技厅基金项目

摘　　要：两种小麦品种绵农4号T r itic u m a e s ti v u m L.c v.M i a n n o n g N o.4和绵农5号T r itic u m a e s ti v u m L.c v.M i a n n o n g N o.5在渐进水分胁迫下相对含水量、质膜相对透性及光系统D C I P2,62二氯酚靛酚光化学还原活性变化的差异显示,绵农5号小麦比绵农4号小麦具有较高的水分胁迫耐受力.进而对比了水分胁迫对这两种耐旱性不同的小麦品种的光系统在基因转录及蛋白代谢等方面的影响.结果表明:渐进水分胁迫下,两种小麦的光系统主要基因p sb A、p sb D、c a b的转录本水平及其编码蛋白D1,D2、L H C的稳态含量分别下降20%及14%以上,而绵农5号c a b基因的转录水平下降程度39.5%与绵农4号61.8%相比明显缓慢,这可能是其具有较高耐旱性的一个重要原因.Two wheat cultivars, Miannong 4( Triticum aestivum L.cv. Miannong No.4 ) and Miannong 5( Triticum aestivum L. cv. Miannong No.5) were used to study relative water content , relative plasmmembrane permeability , DCIP(2,6 dichlorophenol indophenol) photo redution activities in photosystem Ⅱ under progressive stresses. The differences in these regards showed that Miannong 4 and Miannong 5 had relatively high tolerance to the water stresses. Furthermore, the effects of the water stresses on the gene transcript and protein metabolism of photosystem Ⅱ in the two wheat varieties with different tolerances to the water stresses were also studied. The results showed that under the progressive stresses , the transcripts of major genes( psb A, psb D and cab ) and coding proteins (D1,D2, LHCⅡ) of photosystemⅡ decreased by more than 20% and more than 14%, respectively. These reductions became weakened compared with cab transcripts of Miannong 4 and Miannong 5, which was an important reason for relatively high tolerance to water stress.

关 键 词：基因表达 光系统Ⅱ 水分胁迫 小麦 

分 类 号：Q945.78[生物学—植物学] S512.1[农业科学—作物学]