Identification of expressed genes in regenerating rat liver in 0-4-8-12 h short interval successive partial hepatectomy  

作　　者：Cun-ShuanXu Jin-YunYuan Wen-QiangLi Hong-PengHan Ke-JinYang Cui-FangChang Li-FengZhao Yu-ChangLi Hui-YongZhang SalmanRahman Jing-BoZhang 

机构地区：[1]CollegeofLifeScience,HenanNormalUniversity,Xinxiang453002,HenanProvince,China [2]StateKeyLaboratoryofCellDifferentiationandRegulationofHenanProvinceandMinistry,Xinxiang453002,HenanProvince,China [3]HomophiliaResearchCenter,LondonUniversity,LondonSE17EH,UK

出　　处：《World Journal of Gastroenterology》2005年第15期2296-2305,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No. 30270673 and National Key Laboratory Funds, China

摘　　要：AIM: To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles.METHODS: Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray.RESULTS: One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression.Eighty-six genes were unreported, associated with liver regeneration (LR).CONCLUSION: Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar,but the expression of 43 genes is specifically altered in SISPH.AIM:To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression profiles. METHODS: Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by microarray. RESULTS: One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene expression. Eighty-six genes were unreported, associated with liver regeneration (LR). CONCLUSION: Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar, but the expression of 43 genes is specifically altered in SISPH.

关 键 词：SISPH Liver regeneration 

分 类 号：R657.3[医药卫生—外科学]