粉防己中三种双苄基异喹啉生物碱的高效液相色谱法测定  

Determination of Three Bisbenzylisoquinoline Alkaloids in Fourstamen Stephania (Stephania tetrandra) by HPLC

作　　者：何丽一[1] 李宝明[1] 段宏瑾 相楽和彦 大岛俊幸 平山总良 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050 [2]大正制药株式会社,总合研究所

出　　处：《中草药》1994年第12期629-630,共2页Chinese Traditional and Herbal Drugs

摘　　要：建立了同时测定粉防已StephaniatetrandraS．Moore中粉防己碱、去甲粉防己碱及小檗胺等3种双苄基异喹啉生物碱含量的简便精确的高效液相色谱法，用YGW－C1810μm为固定相及甲醇-乙腈-水（3：1：1）含0．06％二乙胺为流动相，种生物碱在10min内可以完全分离。A simple and precise method using high-performane. liquid chromatography was developed for the simultaneous determination of three bisbenzylisoquinoline alkaloids, namely tetrandrine, demethyltetrandrine and berbamine, in Stephania tetrandra S. Moore.A reversed-phase system consisting of chemically bonded ODS silica gel column and methanolacetonitrile-H2O (3: 1:1) containing 0.06% diethylamine as the mobile phase was used. The three alkaloids were completely separated within 10 min. The analytical results for various samples were presented.

关 键 词：粉防己 粉防己碱 去甲粉防己碱 小檗胺 中草药 

分 类 号：R282.710.3[医药卫生—中药学]