84K杨基因转化受体系统的建立  

Establishment of gene transformation receptor system for poplar 84K

作　　者：张岗[1] 康振生[1] 孙燕飞[1] 韩青梅[1] 芦晓飞[1] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2005年第4期87-90,96,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省自然科学基金项目(2004C101)

摘　　要：　以84K杨为研究对象,通过建立叶片再生系统及卡那霉素敏感性试验,确立了稳定高效的基因转化受体系统。结果表明,以MS培养基为基本培养基,0.5～1.0mg/L6-BA+0.01～0.1mg/LNAA组合时,叶片出芽率不到50%,每叶平均生芽数5～6个;1.0～1.5mg/L6-BA+0.02mg/L2,4-D组合时,叶片出芽率达90%～100%,每叶平均生芽数约20个。用后者附加不同质量浓度梯度卡那霉素,确定叶片外植体芽诱导卡那霉素临界筛选质量浓度为20mg/L。利用对84K杨芽生根效果较好的培养基GMS+0.01mg/LNAA+0.25mg/LIBA,附加不同质量浓度梯度卡那霉素,筛选出芽生根卡那霉素临界质量浓度亦为20mg/L。<Abstrcat>Through the establishment of high frequency regeneration system for leaf and kanamycin sensitivity tests,a stable and efficient gene transformation receptor system for poplar 84K was set up,which laid a sound foundation for Agrobacterium-mediated transformation of disease resistance gene by means of leaf disc method.The results indicated that budding percentage was less than 50% and average adventitious buds per leaf was 5-6 when basal MS medium supplemented with 0.5-1.0 mg/L 6-BA+(0.01-)0.1 mg/L NAA.While 1.0-1.5 mg/L 6-BA+0.02 mg/L 2,4-D were added to MS medium,(90%-100%) budding percentage and 20 average adventitious buds per leaf were obtained.Utilizing the latter medium,adding different concentrations of kanamycin,the optimal selecting pressure 20 mg/L of kanamycin for adventitious bud was confirmed.GMS+0.01 mg/L NAA+0.25 mg/L IBA was better to induce root,by which the optimal selecting pressure for root induction was also 20 mg/L of kanamycin for poplar 84K when adding kanamycin different concentrations.

关 键 词：基因转化受体系统 84K杨 卡那霉素 浓度梯度 质量浓度 敏感性试验 基本培养基 MS培养基 叶片外植体 再生系统 生根效果 出芽率 芽诱导 组合 生芽 平均 筛选 临界 

分 类 号：S682.11[农业科学—观赏园艺] S792.11[农业科学—园艺学]