日本血吸虫天然抗原分子的柱层析分离纯化与鉴定  

Purification and Identification of Different Molecular Weight Antigen from Schistosoma japonicum by Column Choromatography

作　　者：彭先楚[1] 汪世平[1] 周松华[1] 姜孝新[1] 吕志跃[1] 徐绍锐[1] 肖小芹[1] 曾少华[1] 余俊龙[1] 戴橄[1] 李文凯[1] 吴仕筠[1] 

机构地区：[1]中南大学湘雅基础医学院病原生物学系血吸虫病研究室,长沙410078

出　　处：《热带医学杂志》2005年第1期12-14,共3页Journal of Tropical Medicine

基　　金：国家"十.五"重大科技专项资助项目(No.2002AA2Z3343);"863"国家血防重大专项(No.2004AA2Z3530);教育部"985行动计划"(No.03-985-3);湖南省"十.五"重点学科建设专项经费(No.2001-207)。

摘　　要：目的分离纯化日本血吸虫成虫(AWA)和未成熟虫卵(SIEA)可溶性抗原中的单一蛋白,为血吸虫病免疫诊断、预防提供新的抗原分子。方法柱层析分离纯化成虫和未成熟虫卵可溶性抗原,SDS-PAGE及银染色进行分析鉴定。结果得到了三种不同大小血吸虫分子抗原AWA18000u,SIEA42000u,SIEA130000u。结论实验证明柱层析法是一种快速分离纯化血吸虫单一组分抗原简单而有效的方法。Objective To obtain the single molecular weight proteins from Schistosoma japonicum adult worm and immature egg soluble antigens for immunodiagnosis and prevention of schistosomiasis. Methods AWA and SIEA were fractionated (purified) by column chromatography, the fractions were analyzed and identified using SDS PAGE and silver staining. Results Three different protein antigens, AWA18 000u, SIEA42 000u, SIEA130 000u, were obtained after the fractionation through column chromatography. Conclusion Above results showed the column chromatography is a simple and effective method for the isolation and purification of some antigens of Schistosoma japonicum.

关 键 词：日本血吸虫 分离纯化 抗原分子 可溶性抗原 未成熟虫卵 天然 免疫诊断 血吸虫病 分析鉴定 PAGE 分子抗原 组分抗原 柱层析法 银染色 SDS 成虫 

分 类 号：R383.24[医药卫生—医学寄生虫学] R284.2[医药卫生—基础医学]