小刺猴头多糖Ⅰ的分离纯化及组成分析  

Study on isolation, purification and composition analysis of HP I from Hericium caput-medusae

作　　者：张金亭[1] 黄勇[1] 李金花[1] 赵丽茹[1] 于文斌[1] 白冬梅[1] 李宇[1] 冷丽[2] 王昭晶[3] 梁忠岩[3] 

机构地区：[1]吉林省生物研究所,吉林长春130012 [2]吉林大学第一临床学院,吉林长春130021 [3]东北师范大学生命科学学院,吉林长春130024

出　　处：《中国药学杂志》2005年第7期545-547,共3页Chinese Pharmaceutical Journal

基　　金：吉林省科技发展计划项目(990559)

摘　　要：目的从小刺猴头菌子实体中提出水溶性粗多糖(HP),从粗多糖中分离出均一多糖并研究其单糖组成。方法DEAE-SepharoseCL-6B及SephadexG-100柱色谱进一步纯化得HPⅠ,柱色谱,醋酸纤维素薄膜电泳等方法检验纯度,GC和PC测其单糖组成。结果HPⅠ为均一多糖,相对分子质量约为65000,单糖组成为:Fuc,Glc,Gal,摩尔比依次为0.423:1:2.110。结论HPⅠ为中性杂多糖,首次从小刺猴头菌子实体中提取获得。OBJECTIVE To isolate homogeneous polysaccharide HP I from HP(a water-solube crude polysacchande from the fruiting body of Hericium caput-medusae) and study the composition of HP I . METHODS HP I was purified by chromatography on DEAE-sepharose(CL-6B) column and sephadex G-100 column. The homogeneity of HP I was proved by column chromatography and film electrophore-sis of cellulose acetate. The composition was studied by GC and PC. RESULTS HP I was homogeneous, and its molecular weight was about 65 000. HP I was composed of fucose, glucose and galactose with the molar ratio of 0.423 : 1: 2.110. CONCLUSION HP I was a neutral heterosaccharide. It was obtained for the first time from the fruiting body of Hericium Caput-medusae.

关 键 词：小刺猴头菌 多糖 分离 组成 

分 类 号：R284.2[医药卫生—中药学]