抗内毒素鸡蛋黄抗体F(ab′)_2制备  

Production of F(ab′)_2 in the chicken egg yolk antibody against lipopolysaccharide

作　　者：史政荣[1] 张雅萍[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,重庆400038

出　　处：《山西医科大学学报》2005年第2期155-158,共4页Journal of Shanxi Medical University

基　　金：中关村医药科技创新基金资助项目(020010)

摘　　要：目的研究抗内毒素鸡蛋黄免疫球蛋白(IgY)用胃蛋白酶切后提取的F(ab′)2片段,探索防治内毒素血症的新途径。方法用内毒素(LPS)作抗原免疫25周龄leghorn鸡,改良水溶法提取抗内毒素IgY,胃蛋白酶切后提取IgY F(ab′)2段,光密度法测抗内毒素IgY F(ab′)2浓度和含量、ELISA检测抗内毒素IgY F(ab′)2效价,SDS-聚丙烯酰胺凝胶电泳检测其分子量及纯度。结果抗内毒素IgY F(ab′)2含量为1.04mg/ml蛋黄液,效价为1∶25600,纯度为90%,相对分子质量为24000。结论抗内毒素IgY F(ab′)2产量大、效价高、特异性强。Objective To explore a new way of preventing and curing endotoximia, research about the F(ab′)_2 in the chicken egg yolk antibody against lipopolysaccharide. Methods Leghorn hens, 25 weeks old, were immunized with lipopolysaccharide . By a simple method of water dilution protein purification, IgY was separated from egg yolk. By the digestion of the pepsin, the F(ab′)_2 were abstracted. It was detected by spectrophotometer and analyzed by SDS-PAGE, and its immunologic competence was determined by enzyme labeling staining reaction (ELISA) . Results IgY-F(ab′)_2 from lipopolysaccharide was found 1.04 mg/ml-egg yolk and 90% purity. Its molecular weight was 24 000. The immunochemistry were 1∶25 600. Conclusion The IgY-F(ab′)_2 against lipopolysaccharide has high titer, well specificity and large production.

关 键 词：内毒素类 免疫球蛋白类 抗体 F(ab’)2 

分 类 号：R392.11[医药卫生—免疫学]