一氧化氮对体外培养大鼠大脑神经细胞的神经毒作用的初探  

A study of neurotoxicity effect of nitric oxide on rat cerebral neuronal cells in culture

作　　者：许丽艳[1] 吕晓红[1] 李恩民[1] 胡亚贤 饶明俐 杨同书[1] 

机构地区：[1]白求恩医科大学应用基础医学研究所

出　　处：《中风与神经疾病杂志》1994年第6期339-341,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：应用细胞培养技术，探讨了大脑神经细胞接触硝普钠（（ＳＮＰ）一氧化氮的供给者）的剂量、时间与神经毒作用的关系，结果显示，接触时间为５ｍｉｎ时，随ＳＮＰ浓度的增高，乳酸脱氢酶（ＬＤＨ）净增加率呈良好的二次回归；而在一定浓度下，随着接触时间的延长，ＬＤＨ净增加率呈良好的线性回归。这些结果表明，一氧化氮（ＮＯ）可产生剂量时间依赖的神经细胞损伤。Abstract We studied the relationship between the neurotoxicity effect and dose, time of sodium nitroprusside(SNP), which spontaneously releases nitric oxide(NO), what cerebral neuronal cells were exposed to. The results showed that SNP concentration being increased, lactate dehydrogenase(LDH) net increased rate is curvilineal regression, when cells were exposed to SNP for 5 min, and in the certain concentration,time of exposure to SNP being prolonged. LDH net increased rate is linear regression. These results suggested that NO can produce dose-time dependent cell death.

关 键 词：一氧化氮 大脑神经细胞 细胞培养 毒性 

分 类 号：R992[医药卫生—毒理学] R329－33[医药卫生—药学]