猪成纤维细胞冻存方法的研究  

Research on Method of freezing fibroblast of pig

作　　者：梁洋[1] 刘娣 孙兴参[2] 周佳勃[2] 岳顺利[2] 于玲珠[1] 

机构地区：[1]东北农业大学动物科技学院 [2]东北农业大学生命科学学院黑龙江哈尔滨150030

出　　处：《黑龙江畜牧兽医》2005年第4期13-14,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省攻关项目(GB01B104)资助

摘　　要：试验以猪的成纤维细胞为研究对象,认为在-70℃情况下冻存至少7d不会影响其存活率(可达80%以上),且生长形态正常。同时利用血清饥饿法使成纤维细胞周期同步化,在-70℃冻存2d、4d和7d,细胞存活率可达92. 3%、87. 2%和83. 6%,甚至在该条件下冻存15d,细胞也能恢复正常的生长。An efficient methed for freezing donor cell is necessary when using nucleus transfer or larger-scale cloning. Cultured cells are usually frozen in liquid nitrogen at -196℃ at random cell cycles. In the study ,fibroblast were frozen at -70℃ for at least 7d and living rate can receive 80%.When fibroblast were serum-stared for three days to induce a quiescent state ,then frozen at -70℃ for 2 d ,4 d and 7 d,the living rate receive 92.3%,87.2%and 83.6%.Thus,storage of fibroblast in the quiescent state at -70℃ is effective and convenient.

关 键 词：冷冻复苏 细胞周期同步化 成纤维细胞 核移植 猪 冻存方法 存活率 

分 类 号：S828.36[农业科学—畜牧学]