不同厂家黄连配方颗粒中盐酸小檗碱、盐酸巴马亭和盐酸药根碱含量比较  

Comparison of berberine hydrochloride,palmtine hydrochloride and jatrorrhizinehydrochloride in dispensing granule of Coptis produced by different factories

作　　者：雷鹏[1] 刘韶[1] 李新中[1] 徐平声[1] 

机构地区：[1]中南大学湘雅医院药剂科,长沙410008

出　　处：《中南药学》2005年第2期90-92,共3页Central South Pharmacy

基　　金：湖南省卫生厅中医药科技基金 (221201)

摘　　要：目的考察不同厂家黄连配方颗粒中盐酸小檗碱、盐酸巴马亭和盐酸药根碱含量.方法用高效液相色谱法测定盐酸小檗碱、盐酸巴马亭和盐酸药根碱含量.色谱柱为Diamonsil C18(4.6 mm×250 mm,5 μm);流动相为0.2 mol·L-1磷酸二氢钠(用磷酸调节pH至3.0)-乙腈(70:30);流速为1 mL·min-1;柱温为30 ℃;检测波长为345 nm.结果不同厂家黄连配方颗粒中盐酸小檗碱含量为3.67～72.53 mg·包-1,盐酸巴马亭含量为0.60～28.70 mg·包-1,盐酸药根碱含量为5.40～26.54 mg·包-1.结论不同厂家产品盐酸小檗碱、盐酸巴马亭和盐酸药根碱含量差异显著.OBJECTIVE To compare the contents of berberine hydrochloride,palmtine hydrochloride and jatrorrhizine hydrochloride by dispensing granule of Coptis produced by different pharmaceutical factories.METHODS The contents of berberine hydrochloride,palmtine hydrochloride and jatrorrhizine hydrochloride were measured by HPLC.Diamonsil C_ 18(250 mm×4.6 mm,5 μm)column was used.The mobile phase was composed of acetonitrile and 0.2 mol·L -1 NaH_2PO_4(adjust pH=3.0 with H_3PO_4)at the rate of 30∶70(v/v).The column temperature was set up at 30 ℃ and the flow rate was 1 mL·min -1.The detection wavelength was 345 nm.RESULTS The content of berberine hydrochloride was from 3.67 to 72.53 mg/package,the content of palmtine hydrochloride was from 0.60 to 28.70 mg/package and the content of jatrorrhizine hydrochloride was from 5.40 to 26.54 mg/package in dispensing granules of Coptis produced by different factories.CONCLUSIONS The contents of berberine hydrochloride,palmtine hydrochloride and jatrorrhizine hydrochloride are obviously different in dispensing granule of Coptis produced by different factories.

关 键 词：黄连配方颗粒 高效液相色谱法 盐酸小檗碱 盐酸巴马亭 盐酸药根碱 

分 类 号：R927.1[医药卫生—药学]