抗除草剂基因基因枪法转化粳稻的研究  

Transformation of Herbicide Resistant Gene to Rice via Biolistic Method

作　　者：王景余[1] 金成海[2] 方秀琴[2] 林秀峰[3] 林秀云[2] 

机构地区：[1]北京师范大学生命科学学院植物发育生理研究室,北京100875 [2]吉林省农业科学院水稻研究所,吉林公主岭136100 [3]吉林省农业科学院生物技术中心

出　　处：《吉林农业科学》2005年第2期3-6,共4页Journal of　Jilin Agricultural Sciences

摘　　要：优化了粳稻的组织培养体系;通过Gus基因的瞬间表达研究,明确了以DNA金弹到靶细胞距离为9cm,轰击2次时,PDS1000/He基因枪法转化水稻胚性愈伤组织的效率最高。应用基因枪法将抗除草剂基因(bar)转入吉林省主栽的水稻品种长白8号中,获得了可育的转基因水稻植株。经过Gus基因的组织化学分析,PCR和Southern杂交检测,证明抗除草剂基因已整合到水稻基因组中得以表达,使水稻产生对除草剂的抗性。转基因水稻中发现一例基因沉默现象。A herbicide gene(bar) was delivered into a commercial rice variety 'Changbai 8' cultivated in Jilin province via biolistic method. Five fertile transgenic rice plants were obtained. Parameters of Gene Gun(PDS-1000/He) transformation of callus derived from mature embryo of Japonica rice variety were optimized by Gus gene transcient expression. When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. It was demonstrated that bar gene had been inserted into rice genome by PCR and Southern hybridization. Three transgenic rice plants (D0-1, D0-2 and D0-3) showed herbicide resistance. Gene silence was observed in one transgenic rice plant (D0-5).

关 键 词：抗除草剂基因 基因枪法转化 粳稻 Southern 转基因水稻植株 Gus基因 组织培养体系 胚性愈伤组织 水稻基因组 瞬间表达 水稻品种 化学分析 杂交检测 基因沉默 靶细胞 DNA 吉林省 PCR 长白 抗性 

分 类 号：S512.1[农业科学—作物学] S511.035