西农萨能奶山羊乳腺上皮细胞体外培养  

In Vitro Culture of Mammary Epithelial Cells in Goat

作　　者：高亚军[1] 唐爽[1] 孙苏军[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《安徽农学通报》2005年第2期74-75,共2页Anhui Agricultural Science Bulletin

摘　　要：用无菌手术法取健康的泌乳期西农萨能奶山羊的乳腺组织,用组织块法进行体外条件下的培养。基础培养液为D-MEM/F-12,含10%胎牛血清,添加EGF(0.01mg/L)、氢化可的松(1mg/L)、孕酮(1mg/L)、胰岛素-转铁蛋白-硒钠(5mg/L)、青霉素(0.05g/L)及链霉素(0.05g/L)。结果表明,获得良好的山羊乳腺上皮细胞原代培养物,传代培养细胞增殖旺盛,长势良好。The mammary tissues from healthy xinong saanen goat during lactation have been cultured in vitro. The basic medium is D-MEM/F-12 and 10% fetal calf serum,and EGF(0.01mg/L),hydrocortisone (1mg/L),P(1mg/L), ITS(5mg/L),penicillin(0.05g/L) and streptomycin(0.05g/L) were added.The results showed that the goat mammary epithelial cell grows well and proliferates vigorousely.

关 键 词：乳腺上皮细胞 西农萨能奶山羊 体外培养 

分 类 号：S827[农业科学—畜牧学]