轻度修饰低蜜度脂蛋白对小鼠肝脾中清道夫受体mRNA表达的影响  

Effects of Mildly Modified Low Density Lipoprotein on the Expression of Scavenger Receptor mRNA in Mouse Liver and Spleen

作　　者：姜传仓 陈兵[1] 范乐明[1] 邵莉君 蔡海江[1] 

机构地区：[1]南京医科大学动脉粥样硬化研究中心

出　　处：《中国动脉硬化杂志》1994年第4期129-132,共4页Chinese Journal of Arteriosclerosis

摘　　要：小鼠静脉注射轻度修饰低密度脂蛋白（ｍｉｌｄｌｙｍｏｄｉｆｉｅｄｌｏｗｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ｍｍ－ＬＤＬ）可使其肝、脾清道夫受体（ｓｃａｖｅｎｇｅｒｒｅｃｅｐｔｏｒ，ＳＲ）ｍＲＮＡ水平分别比静脉注射天然ＬＤＬ小鼠增加２．０倍（Ｐ＜０．０５）及５．９倍（Ｐ＜０．０１）；巨噬细胞克隆刺激因子（ｍａｃｒｏｐｈａｇｅｃｏｌｏｎｙ－ｓｔｉｍｕｌａｔｉｎｇｆａｃｔｏｒ，ＭＣＳＦ）ｍＲＮＡ水平亦分别增加３．２倍（Ｐ＜０．０１）和３．０倍（Ｐ＜０．０１）。而静脉注射ＭＣＳＦ时又可使其肝脾ＳＲｍＲＮＡ分别增高３．６倍（Ｐ＜０．０５）及３．０倍（Ｐ＜０．０１），并有一定的量效关系。首次报道了ｍｍ－ＬＤＬ静脉注射可直接诱导肝脾中ＳＲｍＲＮＡ表达，或间接通过刺激ＭＣＳＦ表达，进而诱导ＳＲｍＲ－ＮＡ表达，并讨论了此现象在全身及局部动脉粥样硬化形成中的意义。Compared with giving native low density lipoprotein(LDL) intravenously,scavenger receptor(SR)mRNA levels in liver and spleen of the mice given mildly modified LDL(mm-LDL)intravenously increased 2. 0 times (P<0. 05)and 5.9 times(P<0. 01),respectively;and macrophage colony-stimulating factor(MCSF)mRNA levels also increased 3.2 times(P<0. 01) and 3. 0 times P<0. 01) respectively.Giving MCSF intravenously to mice increased SR mRNA levels in liver and spleen by 3. 6 times(P<0. 05) and 3. 0 times (P<0.01),respectively, and showed some dose-effect relationship.It suggests that giving mm-LDL intravenously might directly induce the expression of SR mRNA in liver and spleen,orindirectly stimulate the expression of MCSF,which in turm induces SR mRNA expression,The significance of these phenomena for local atherogenesis is discussed.

关 键 词：低密度脂蛋白 巨噬细胞 核糖核酸 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病] R362[医药卫生—内科学]