高效液相色谱法测定复方消毒剂中三氯生含量观察  

OBSERVATION ON MEASUREMENT OF TRICLOSAN IN COMPOUND DISINFECTANT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：洪麟 傅振华[2] 洪传沪 

机构地区：[1]上海麟翔生物技术有限公司,上海200063 [2]上海市疾病预防控制中心

出　　处：《中国消毒学杂志》2005年第2期179-180,共2页Chinese Journal of Disinfection

摘　　要：采用高效液相色谱法测定复方消毒剂中三氯羟基二苯醚(三氯生)含量,对该方法进行了观察。结果,在C18色谱柱和甲醇-水流动相条件下,用高效液相色谱法测定复方消毒剂中三氯生,定量范围为3.0～250.3μg/ml,回收率为99.85～100.43%,最低检测量为0.3μg/ml。结论,高效液相色谱法测定复方消毒剂中三氯生含量,定量范围宽,回收率高,专一性好。High performance liquid chromatography (HPLC) was used to measure triclosan content in compound disinfectant in order to observe this method. Results: Under conditions of C_ 18 chromatographic column and methanol-water as the mobile phase, HPLC was used to measure triclosan in compound disinfectant. The quantitative range was 3.0～250.3 μg/ml, recovery rate was 99.85%～100% and the minimum detectable content was 0.3μg/ml. Conclusion: Measurement of triclosan in compound disinfectant by HPLC has the advantages of wide quantitative range, high recovery rate and good specificity.

关 键 词：三氯生 高效液相色谱 复方消毒剂 

分 类 号：R187[医药卫生—流行病学]