引起番茄坏死病的黄瓜花叶病毒TN分离物的研究  

Studies on the TN Strain of Cucumber Mosaic VirusInducing Tomato Necrosis Discase

作　　者：程宁辉[1] 濮祖芹[1] 方中达[1] 

机构地区：[1]南京农业大学植保系

出　　处：《中国病毒学》1994年第2期143-150,共8页Virologica Sinica

摘　　要：从南京郊区田间番茄坏死病株中分离出黄瓜花叶病毒ＴＮ分离物，经人工接种１０科３９种植物，能侵染其中的８科２６种，接种番茄，辣椒和普通烟都引起坏死症状。ＴＮ分离物的失毒温度为５０℃，稀释限点５×１０￣（－３）－５×１０￣（－４），体外存活期１－２天。蚜虫以非持久性方式传毒，经免疫双扩散测定，ＴＮ分离物与黄瓜花叶病毒抗血清呈阳性反应。用差速离心提纯的病毒粒子经电镜观察为廿面体，直径为２８ｎｍ。ＳＤＳ－ＰＡＧＥ分析病毒外壳蛋白为单一组份，分子量２７５００Ｄ。病毒核酸组份分析发现，该病毒分离物含有ＣＭＶ基因组核酸和一个低分子量的卫星ＲＮＡ。以上结果同已报道的引起番茄坏死的带卫星ＲＮＡ的黄瓜花叶病毒株系一致（Ｋｅａｒｎｅｙ，Ｃ．Ｍ．１９９０；Ｊｏｒｄａ，Ｃ．１９９２）。本文还讨论了该病害的发生和流行。lethal disease of tomato eecurred severely in the vicinity of Nanjing from 1991 to 1992 wascaused by a viral pathogen.A virus was isolated from the diseased tomato collected from field and.called the isolate TN.It infected 8 families 26 species host plants out of 10 families 39 species inoculated in the host ranse test and induced necrosis in tomato, pepper and tobacco.Its stability in extractedsap was TIP 50℃,DEP 5×10-3-10-4,and LIV 24-48hrs.The virus transmitted by aphid in non-persistence and reacted positively with CMV antiserum in agar diffusion test.Purified virions of the isolate TN were obtained by differential centrifugation and its morphology was spherical about 28nm in diameter by electron microscopy.Analysis of SDS-PAGE revealed that virion capsid protein had a singlecomponent (monomer),which molecular weight was 27500D.Viral RNAs contained normal CMVRNA 1-4 and additional lower molecular weight RNA5 by polyacrylamide gel electrophoresis. Theseresults were concurrent with the previous reports that CMV strains associated with satellite RNA induced tomato necrosis(Kearney C M,1990:Jorda C,1992),so it was suggested that TN be thenecrotic isolate of CMV associated with satellite RNA.

关 键 词：番茄 黄瓜花叶病毒 TN分离物 

分 类 号：S432.41[农业科学—植物病理学]