生物素标记HBV RNA探针的制备及应用  

Preparation of Biotin HBV RNA Probe andIts Appllcation in Serum Detection

作　　者：陈晓旭[1] 侯淑霞 李若伦[1] 

机构地区：[1]沈阳军区大连医学高等专科学校,北京福瑞生物工程公司,吉林医学院附属医院免疫室

出　　处：《中国病毒学》1994年第2期95-99,共5页Virologica Sinica

摘　　要：本文首次采用ＳＰ＿（６５）特殊质粒与人类的乙型肝类病毒ＤＮＡ（ＨＢＶＤＮＡ）重组，制备了Ｂｉｏ－ＨＢＶＲＮＡ探针，能特异地与ＨＢＶＤＮＡ杂交。将该探针与缺口转移（ＮｉｃＨｒａｎａｌａｔｉＯｎ）方法标记的ｍ小ＨＢＶＤＮＡ探针进行了比较。结果显示出Ｂｉｏ－ＨＢＶＲＮＡ探针比Ｂｉｏ－ＨＢＶＤＮＡ探针的敏感性提高１０倍。并分别应用两种探针同时检测７０例己肝病人血清中ＨＢＶＤＮＡ，阳性率各为３１．４２％、２８．５７％（Ｐ＞０．２５）．对Ｂｉｏ－ＨＢＶＲＮＡ探针应用于临床检测乙肝病毒ＤＮＡ的效能也进行了初步探讨。epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP_(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection of HBV DNA,HBVRNA probe are 10 times sensitive than that of HBV DNA.In addition,70 serum saniples of hepatitis B potients have been detected by hybridization withtwo probes.Pooitive proportion of HBV DNA corresponils to 31.42%, 28.57%(P>0.25).The clinical application of HBV RNA probes was discusseil preluninarily.

关 键 词：生物素探针 乙型肝炎病毒 RNA 

分 类 号：R512.620.3[医药卫生—内科学]