中国对虾中一种球状病毒的分离提纯与检测  

Isolation and Detection fo Spherical Viruses from Penaeus orientalis K

作　　者：陈棣华[1] 张建红[1] 石正丽[1] 高玮[1] 高学兴 吴志广 

机构地区：[1]中国科学院武汉病毒研究所,河北省乐亭县水产局

出　　处：《中国病毒学》1994年第2期170-173,共4页Virologica Sinica

基　　金：国家自然科学基金

摘　　要：从人工养殖生长比较缓慢的对虾与病虾的肝胰腺和消化道中，分离出一种大小为８０ｎｍ的球状病毒。同时采用超薄切片技术可在对虾中肠细胞中发现大量的球状病毒，这些病毒聚集成片，在同一细胞内的细胞器，如线粒体等均发生病变。此外，将分离提取的球状病毒进行感染试验，感染死亡率为６０％。并且从感染致死的对虾消化腺及肠道中，同样可以观察到大小相同、形态一致的球状病毒颗粒。uring the cultivition of Penaeus orientalis K,outbreak of death or slowing down of growth couldoccurred sometimes.tuge amount of dispersive spherical viruses with diameter around 80nm were observed in the midgut cells of those sick shrimps under electron microscope.Hoat cell organelles,such asmitochondria,became over swollen,and even broken.The virus was isolated from the hepotopancreasand digestive tube of the sick shrimp and the viriolls were icosahedron with diameter of 80nm on the av-erage.From infection experiment the corrected mortality was as high as 60%.The salne morphological spherical virus with the same size was also isolated from infected died shrimps digeetive glands andtubes.

关 键 词：中国对虾 肝 胰腺 球状病毒 提纯 

分 类 号：S917.1[农业科学—水产科学]