戊型肝炎病毒细胞分离株部分核苷酸序列分析  

Partial Genome Sequence Analysis of Hepatitis E Virus Isolated by Cell Culture

作　　者：陈万荣[1] 田辛[1] 鲍作义[1] 李敬云[1] 邓大荣[1] 

机构地区：[1]北京军事医学科学院微生物流行病研究所,新疆军区军事医学研究所

出　　处：《中国病毒学》1994年第3期213-216,共4页Virologica Sinica

基　　金：世界卫生组织资助课题

摘　　要：用抗原捕获／多聚酶链反应（ＡＣ／ＰＣＲ）对戊型肝炎病毒（ＨＥＶ）细胞分离株ＭＪ９０和Ｒ２５基因组的部分核苷酸序列进行扩增，获得了与ＨＥＶ缅甸株ＥＴ１·１相同的ｃＤＮＡ扩增带。该ｃＤＮＡ扩增带纯化后用双脱氧核苷酸ＤＮＡ链末端终止法测序，ＣＪ９０、Ｒ２５株的核苷酸和氨基酸序列与ＥＴ１·１克隆的同源性分别为９９．６％、１００％和９９％、９９％，从而证明ＭＪ９０和Ｒ２５毒株为ＨＥＶ．enome of presumed hepatitis E virus XJ90 and R25 was partially amplified by antigen capturepolymerase chain reaction(AC/PCR).cDNA band same as ET1·1 clune was ubserved.After purification,this cDNA band was sequenced using dideoxy mediated chain termination method.The nu-cleotide and amino acid homology between XJ90 R25 and ET1·1 clone were 99.6%,100%and99%,99%,respectively. This result showed that both XJ90 and R25 are hepatitis E virus.

关 键 词：戊型肝炎病毒 核苷酸 序列 分析 

分 类 号：R373.21[医药卫生—病原生物学]