淋巴细胞内pHi,Na^＋—H^＋交换活性与SHRSP关系的研究  

A study on the relationship between pH, Na￣+-H￣+ exchange activity in lymphocytes from SHRSP

作　　者：李迪元[1] 小林绫女 

机构地区：[1]上海第二医科大学上海市高血压研究所

出　　处：《中国病理生理杂志》1994年第1期55-58,共4页Chinese Journal of Pathophysiology

摘　　要：本文应用ｐＨ敏感的ＢＣＥＣＦ和Ｃａ敏感的Ｆｕｒａα萤光染料，分别测定雄性。１６周龄ＳＨＲＳＰ和ＷＫＹ循环淋巴细胞ｐＨｉ，Ｎａ￣＋－Ｈ￣＋交换活性和［Ｃａ￣（２＋）］ｉ，结果表明ＳＨＲＳＰ循环淋巴细胞ｐＨｉ、Ｎａ￣＋－Ｈ￣＋交换活性和［Ｃａ￣（２＋）］ｉ均显著高于ＷＫＲ，而细胞缓冲能力在两组大鼠之间没有显著差异。提示在ＳＨＲＳＰ细胞膜可能存在Ｎａ￣＋，Ｈ￣＋交换遗传性缺陷。将两组大鼠的资料合并，经直线回归相关分析发现ｐＨｉ与Ｎａ￣＋－Ｈ￣＋交换活性和ｐＨｉ与［Ｃａ（２＋）］ｉ均呈显著正相关。表明Ｎａ￣＋－Ｈ￣＋交换活性和［Ｃａ￣（２＋）］ｉ参与ｐＨｉ调节．它们之间既相互调节又相互制约。细胞内Ｎａ、Ｃａ和细胞膜Ｎａ￣＋－Ｈ￣＋离子转运系统异常可能是高血压病因中重要因素之一。pH_i, Na￣+-H￣+ exchange activity and[Ca￣2]_i in circulating lymphocytes fromSHRSPs and WKYs were measured with pH-sensitive and Ca-sensitive fluorescent dye,BCECF and Fura 2. The results suggested that pH_i,Na￣+-H￣+ exchange activity and[Ca￣(2+)]_iwere much higher in SHRSPs than in WKRs.Buffering power of the cells form the twostrains were not difference.When data from both groups were combined, there showedthe fllowing statistically significant correlations: pH_i and H￣+ efflux rate(r=0.471,n=41,P<0.01); pH_i and [Ca￣(2)]_i(r=:0.416,n=32,P<0.05).These findings seemed toindicated that pH-i was regulated not only by Na￣+-H￣+ exchange but also by [Ca￣(2+)]_i ,andthe abnormal cellular pH_i, Na￣+-H￣+ exchange activity and [Ca￣(2+)]_i which existed in SHRSPmight have some etiological link with inheritance of hypertension.

关 键 词：钙 高血压 钠离子 钾离子 交换活性 

分 类 号：R544.102[医药卫生—心血管疾病]