珠子参总皂甙、三七总皂甙及绞股兰总皂甙对心肌缺血再灌损伤的保护作用  

Protective effects of panax notoginseng saponins(PNS), panax Japonicus saponins ( PJS )and gypenosides(GP)on myocardial ischemia and reperfusion injury

作　　者：韩红[1] 李麟仙[1] 王琼仙[1] 魏均娴[1] 陈业高[1] 

机构地区：[1]昆明医学院病理生理教研室,昆明医学院植化研究室

出　　处：《中国病理生理杂志》1994年第2期128-133,共6页Chinese Journal of Pathophysiology

摘　　要：采用Ｌａｎｇｅｎｄｏｒｆｆ离体心脏灌流方法，以停灌３０分钟，再灌注２０分钟制备大鼠心肌缺血再灌损伤模型。三七总皂甙（ＰＮＳ３０ｍｇ／Ｌ）和绞股兰总皂甙（ＧＰ，３０ｍｇ／Ｌ和５０ｍｇ／Ｌ）显著降低不可逆室颤发生率，５０ｍｇ／Ｌ的ＧＰ显著抑制心肌ＬＤＨ释放，ＰＮＳ和ＧＰ显著提高缺血再灌心肌ＳＯＤ活性，ＧＰ明显降低其ＭＤＡ水平，ＰＮＳ明显降低心肌钙含量。在结扎家兔冠状动脉左室支２小时，再灌注３０分钟所造成的心肌缺血再灌损伤模型中，珠子参总皂甙ＰＪＳ，２５０ｍｇ／ｋｇ）和ＰＮＳ（２００ｍｇ／ｋｇ）轻度改善缺血再灌后的左室功能，显著降低再灌后血浆ＬＤＨ和ＣＰＫ水平，明显减轻心肌钙聚积。以上结果表明：ＰＪＳ，ＰＮＳ和ＧＰ对心肌缺血再灌损伤具有保护作用，ＰＪＳ和ＰＮＳ的作用机理可能与拮抗钙有关，ＧＰ的作用机理可能与抗脂质过氧化有关。Myocardial injury of isolated perfused rat heart was induced by 30 min global ischemia and 20 min reperfusion. It was found that PNS(30 mg/L)and GP(30 mg/L or 50 mg/L lowered the irrevisible VF rate. GP ( 50 mg/L supressed the release of LDH from rat heart at reperfusion significantly.In addition, PNS alleviated the reper fused myocardial calcium accumulation markedly and GP reduced myocardial MDA con tent obviously. Both PNS and GP elevated the reperfused myocardial SOD activity. In an other model, myocardial injury was produced in rabbit by ligation of left ventricular coro nary artery for 2 br folloWed by 30 min reperfusion, It was found that PNS(200 mg/k8)and PJS(250 mg/kg )partially ameliorated left venticular functions and reducedplasmal LDH and CPK level very much at reperfusion. Calcium accumulation in the re perfused Myocardium was much lessened. These results suggested that PNS, PJS and GPpossess protective effects on myocardial damag caused by ischemia and reperfusion. It alsosuggested that the underlying protective mechanisms of PJS and PNS are related to theprevention of calcium overload, and that GP has an action of anti lipid peroxidation.

关 键 词：心肌缺血 再灌注 人参 皂甙 三七 

分 类 号：R259.422[医药卫生—中西医结合]