低剂量辐射刺激小鼠抗体形成机制的研究  

Mechanism of stimulative effect of low dose irradiation on antibody formation in mice

作　　者：刘伟宏[1] 龚守良[1] 刘树铮[1] 

机构地区：[1]白求恩医科大学放射生物教研室

出　　处：《中国病理生理杂志》1994年第3期235-240,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：本研究检测了单次Ｘ射线和连续γ射线全身照射后小鼠脾脏抗体形成反应、胸腺细胞氚标胸腺嘧啶（￣３Ｈ－ＴｄＲ）自发掺入率、脾细胞白介素－２（ＩＬ－２）形成和脾脏抑制性Ｔ细胞（Ｔｓ）功能的变化。结果表明，单次Ｘ射线照射７５ｍＧｙ后，免疫小鼠抗体形成反应增强，胸腺细胞自发增殖能力增高，脾细胞ＩＬ－２形成增多，而脾脏和胸腺中抑制性Ｔ细胞功能未见改变。连续γ射线照射累积６５ｍＧｙ所得结果与单次Ｘ射线照射相似。说明低剂量辐射对抗体形成的刺激作用并非由于Ｔｓ功能减弱所致，而可能和辅助性Ｔ细胞（Ｔ＿Ｈ）的激活及ＩＬ－２形成增多有关。C 57BL/6 mice and Kunming mice were whole-body irradiated with single75 mGy X-ray or cumulative 65 mGy γ-ray and immunized with SRBC.The antibodyforming reaction Of splenocytes and a series of related immunological parameters were ana-lyzed 4 and 7 days after irradiation.It was found that after single X-irradiation the anti-body forming reaction of splenocytes was stimulated significantly and IL-2production bysplenocytes was increased markedly.Not much changes in the suppressor T cell activitywere found in both spleen and thymus.The same reaction tendency was found after chro-nic γ-irradiation with cumulation dose of 65 mGy.These data suggested that the stimula-tive effect of antibody formation following low dose irradiation was not caused by prefer-ential deletion of the Ts.The stimulated T_H and augmentation of IL-2 production maylead to an increase of antibody formation.

关 键 词：辐射 抗体形成 白细胞介素2 

分 类 号：R811.5[医药卫生—放射医学]