荷人鼻咽癌裸鼠血清丙二醛、自然杀伤细胞活性以及中药＂癌克星＂的影响  

Serum MDA, splenic NK activation in nude mice bearing human NPC and effects of "Ai Ke Xing

作　　者：杨惠玲[1] 黄静姝[1] 简志瀚[1] 马志楷[1] 陈宏智[1] 潘伟雄[1] 朱振宇[1] 

机构地区：[1]中山医科大学病理生理教研室

出　　处：《中国病理生理杂志》1994年第3期258-261,共4页Chinese Journal of Pathophysiology

摘　　要：本实验用ＢＡＬＢ／ｃ裸小鼠复制人ＮＰＣ模型，检测荷人ＮＰＣ裸鼠血清ＭＤＡ和脾ＮＫ活性，以探讨瘤宿主ＮＫ活性改变的原因和可能机理，观察中药“癌克星”制剂对ＮＰＣ的抑瘤作用及对ＭＤＡ含量和脾ＮＫ活性等的影响；以探讨其抑瘤机理。结果发现荷人ＮＰＣ裸鼠血清ＭＤＡ含量（３７．９２±５．３６ｎｍｏｌ／ｍｌ）明显高于正常裸鼠（１３．９０±１．６３ｎｍｏｌ／ｍｌ）（Ｐ＜０．０１），而脾ＮＫ活性（４０．９３±２．３９）则明显低于正常裸鼠（６７．０９±６．２８）（Ｐ＜０．０１）。中药“癌克星”制剂对荷瘤鼠具明显抑瘤作用，抑瘤率２５．８５～９９．９９％；并可降低荷瘤鼠血清ＭＤＡ含量，部分提高ＮＫ活性，增加瘤组织单核细胞的浸润。本实验提示，ＦＲ可能作为免疫抑制因子之一，使ＮＫ活性下降。“癌克星”对ＮＰＣ具明显抑癌效应，其抑瘤作用可能是通过降低瘤宿主体内ＦＲ，提高机体免疫功能而实现。BALB/c Nude mica(NM)bearing nasopharyngeal carcinoma(NPC )was reproduced by hypodermic implanting human CNE-2 cell into right-side of axillaryfossa The change of serum malondialdehyde(MDA)and splenic NK bearing humanNPC were examined.Meanwhile,we observed the effected of "Ai Ke Xing"on tumor'ssize,serum MDA and splenic NK activation in NM bearing human NPC.Results indicated that in NM bearing human NPC serum MDA content(37.92 ± 5.36 nmol /ml) increasedmarkedly, while splenic NK activation decreased significantly, in comparison with thecontrol group (MDA 13.90± 1.63 nmol/ml,splenic NK cativation 67.09± 6.28). "Ai KeXing" inhibited NPC growth.Rate of inhibition was between 2 5.8 5 and 9 9.99 per cent.It decreased serum MDA,elevated splenic NK activation and enhanced mononuclears toinfiltrate to the transplanted NPC tissue.These results suggested that free radicals mightfunction as one of the immunosupressive factors.It might cause host's splenic NKactivation decreasing. "Ai Ke Xing" had antitumor effect.It might have decreased freeradicals and elevated splenic NK activation in NM bearing human NPC.

关 键 词：癌 自由基 鼻咽肿瘤 中医药疗法 

分 类 号：R273.963[医药卫生—中西医结合]