糖尿病大鼠血清一氧化氮及其抗氧化功能的变化  

Changes of serum nitric oxide level and antioxidative functions in diabetic rats

作　　者：吴蕴棠[1] 车素萍[1] 孙忠[1] 常红[1] 王永明[1] 

机构地区：[1]天津医科大学公共卫生学院,天津300070

出　　处：《天津医科大学学报》2005年第1期11-14,共4页Journal of Tianjin Medical University

基　　金：天津市自然科学基金资助(编号:993606611);天津医科大学青年基金资助(编号:98Q09)

摘　　要：目的:观察糖尿病大鼠血清一氧化氮(NO)及其抗氧化酶的改变,以探讨NO及其抗氧化系统的变化对胰岛细胞病理损伤的影响。方法:雄性Wistar大鼠22只,随机留取12只为正常对照组,其余大鼠以四氧嘧啶诱导成糖尿病模型,为糖尿病组。分别进行实验前后生化指标的检测及胰岛细胞的形态学观察。结果:糖尿病大鼠红细胞超氧化物歧化酶(SOD)、全血谷胱甘肽过氧化物酶(GSH-Px)活性随病程的延长由升高至降低,8周末,明显低于正常对照组,同时血清MDA水平高于正常对照组;血清NO及一氧化氮合酶(NOS)随病程的延长逐渐升高,8周末,明显高于正常对照组。结论:糖尿病大鼠血清NO的升高、抗氧化系统平衡失调可能是其胰岛细胞病理损伤的原因之一。Objective: In order to observe the changes of serum nitric oxide level and antioxidative functions and to study their effects on pathological damage of pancreatic islets in diabetic rats. Methods: 12 rats were randomly selected from 22 male Wistar rats as normal control group (NC group).The rest were induced by alloxan into diabetic model as DM group. At the end of the experiment, the blood biochemical analysis and pancreatic islets morphological observation were performed. Results: The activities of SOD, GSH-Px in DM group were increased at 2nd week, but obviously lower than those of NC group at 8th week. Compared with NC group, the levels of serum MDA, NO, NOS levels in DM group were significant higher at 8th week. Conclusion: The increase of serum NO level and imbalance of antioxidant system may be one of the reasons for pathological damage of pancreatic islets in diabetic rats.

关 键 词：糖尿病 抗氧化酶 一氧化氮 自由基 

分 类 号：R587.1[医药卫生—内分泌]