HPLC法测定地红霉素片含量  

The determination of Contents of Dirithromycin Tablets by HPLC

作　　者：刘新元[1] 

机构地区：[1]天津隆顺榕制药厂,天津300232

出　　处：《天津医科大学学报》2005年第1期116-117,共2页Journal of Tianjin Medical University

摘　　要：目的:建立高效液相色谱法测定地红霉素片的含量。方法:色谱柱为C18,以磷酸盐缓冲液(取磷酸二氢钾1.41g和磷酸氢二钾6.91g,加水至1000ml,使溶解)乙腈-甲醇(20∶55∶25)为流动相,流速1.1ml/min,检测波长为205nm。结果:地红霉素浓度在0.2～1.0mg/ml范围内,线性良好,相关系数r=0.9996(n=5),平均回收率为99.5%,日间和日内RSD分别为0.6%和0.7%。结论:方法简便、准确、专属。Objective: To establish RP-HPLC method to determine the Contents of Dirithromycin tablets. Methods: In the method C18 (250 mm×4.6 mm,5 μm; is used as the column and phosphate buffer solution(1.41g KH2PO4 and 6.91g K2HPO4 in 1000ml water;-actronitrile-methanol (20∶55∶25; as the mobile phase and the flow rate of 1.10ml/min and the detection wavelength of 205 nm was specified. Results: The standard curve for Dirithromycin was liner in the range 0.2～1.0mg/ml(r=0.999 6,n=5;,the average recovery rate of Dirithromycin was 99.5%,The intra-day and inter-day RSD was 0.6% and 0.7% respectively. Conclusion: The method is convenient, sensitive and actuate.

关 键 词：高效液相色谱法 地红霉素片 含量 

分 类 号：R695.2[医药卫生—泌尿科学]