离子对反相高效液相色谱法同时测定复方氟脲嘧啶二甲亚砜溶液中氟脲嘧啶和氢化可的松的含量  

Simultaneous assay of fluorouracil and hydrocortison in compound fluorouracil and dimethy sulfoxy solution by ion-pair RP-HPLC method

作　　者：杜静 傅应华[2] 

机构地区：[1]浙江杭州红十字会医院,浙江杭州310009 [2]浙江嘉兴学院医学院,浙江嘉兴314001

出　　处：《中国现代应用药学》2005年第2期163-165,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立RP-HPLC测定复方氟脲嘧啶二甲亚砜溶液中氟脲嘧啶和氢化可的松的含量.方法以水为溶剂,采用RPHPLC法,以Supelco discovery C18(150mm×4.6mm,5μm)为色谱柱,甲醇-0.5%醋酸(内含0.005mol/L庚烷磺酸钠)(60:40)为流动相,检测波长为248nm.结果氟脲嘧啶浓度在61.6～143.8μg·mL-1与氢化可的松浓度在6.3～14.6μg·mL-1之间与峰面积呈良好线性关系,日内、日间精密度(RSD)氟脲嘧啶为0.52%和0.63%,氢化可的松为0.49%和0.76%,平均回收率(n=6)氟脲嘧啶为(101.0±0.32)%,氢化可的松为(100.1±0.23)%.结论本法简便、快速、结果准确,适于该制剂的质量控制.OBJECTIVE To establish an ion-pair RP- HP LC method for determination of fluoruracil and dimethy sulfoxy in compound fluor ouracil and dimethy sulfoxy solution.METHOD Analytical column was discovery C 18(150mm×4.6mm,5 μm),Mobile phase consisted of methanol -0.5% acetic acid (contained 0.005mol ·L -1 of heptanesulfonic sodium) (60∶40),detection wavelength was 248nm.RESULTS The linearities were obtained in the ranges of 61.6～14 3.8μg·mL -1 (r=0.9998) from fluorouracil and 6.3～14.6μg·mL -1 (r=0.9999) from hydrocortisone.The precisions (RSD) of intra-day and inter -day were 0.52% and 0.63% from fluorouracil and 0.49% and 0.76% from hydroc ortisone.The mean recoveries (n=6) were (101.0±0.32)% fro fluorouracil and (100.1±0.23)% for hydrocortisone.CONCLUSION The method is simple,accurate,rapid and suitable for the quality control of this preparation.

关 键 词：离子对反相高效液相色谱法 氢化可的松 复方氟脲嘧啶 二甲亚砜 同时测定 含量 溶液 RP-HPLC测定 HPLC法 平均回收率 线性关系 检测波长 质量控制 色谱柱 流动相 磺酸钠 峰面积 精密度 浓度 

分 类 号：R974.2[医药卫生—药品] R977.11[医药卫生—药学]