慢性乙型肝炎患者PMN内游离钙含量与PMN凋亡的研究  

Study of intracellular calcium concentration and the apoptosis of polymorphonuclear in patients with chronic hepatitis

作　　者：单容[1] 王爱光[2] 林森[3] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东省千佛山医院 [3]山东大学第二医院

出　　处：《山东医药》2005年第10期4-5,共2页Shandong Medical Journal

基　　金：济南市青年科技明星计划资助项目(No.40104)

摘　　要：目的了解慢性乙型肝炎患者外周血多形核粒细胞(PMN)内游离钙含量变化、凋亡情况及其在慢性乙型肝炎发病机制中的作用。方法分离正常外周血PMN加入乙肝患者血清,在体外培养0、3、6、12小时后负载荧光染料用激光扫描共聚焦显微镜测定PMN内游离钙含量,同时应用流式细胞仪检测PMN凋亡情况。结果PMN内游离钙含量及凋亡率较正常对照均降低。结论慢性乙型肝炎患者外周血PMN凋亡延迟。Objective To investigate the change of intracellular calcium concentration and the apoptosis of polymorphonuclear(PMN) in the peripheral blood of patients with chronic hepatitis B.Methods PMN was isolated from the peripheral blood of healthy donors.Before loading fluorescence,PMN was cultivated in vitro and for 0,3,6 and 12 hours respectively.Then the concentration of intracellular free calcium was measured with laser scanning confocal microscopy(LSCM) and the apoptosis of PMN was measured with flow cytometry.Results The concentration of intracellular free calcium and the rate of apoptosis of PMN in the peripheral blood of test groups were less than the control groups.Conclusion The apoptosis of PMN in the peripheral blood of hepatitis B patients may delay.

关 键 词：慢性乙型肝炎患者 PMN凋亡 钙含量 流式细胞仪检测 多形核粒细胞 显微镜测定 外周血 含量变化 发病机制 乙肝患者 体外培养 激光扫描 荧光染料 正常对照 游离钙 共聚焦 凋亡率 

分 类 号：R512.62[医药卫生—内科学] R726.92[医药卫生—临床医学]