HPLC法同时测定感冒喷雾剂主药含量  

Determination of naphazoline hydrochloride and chlorphenamine maleatein cold nasal spray by RP-HPLC

作　　者：颜晗[1] 陈静芬[1] 李玉兰[1] 

机构地区：[1]深圳市药品检验所,深圳518029

出　　处：《天津药学》2005年第2期19-21,共3页Tianjin Pharmacy

摘　　要：目的:建立同时测定感冒喷雾剂中盐酸萘甲唑林和马来酸氯苯那敏含量的HPLC法。方法:采用ODS柱,以乙腈-1.0%十二烷基硫酸钠-冰醋酸(65∶35∶0.3)为流动相,检测波长为264nm。结果:盐酸萘甲唑林Y=19132.4X+11486.8,r=0.9999(n=5),线性范围15.18～25.31μg/ml,回收率99.6%;RSD0.60%(n=5)。马来酸氯苯那敏Y=14214.3X+45201.5,r=0.9999(n=5),线性范围76.56～127.6μg/ml,回收率99.8%。RSD0.63%(n=5)。结论:本法方便、快捷、准确、精密度高,适用于感冒喷雾剂的含量测定。0bject: To establish an RP-HPLC method for the determination of naphazoline hydrochloride and chlorphenamine maleate in cold nasal spray. Methods: A ODS column was used for the separation. The mobile phase was consisted of acetonitrile-1.0% sodium dodecyl sulfate-glacial acetic acid (65∶35∶0.3) and the detection wavelength was 264nm. Results:The linear range was 15.18~25.31 μg/ml for naphazoline (r=0.999 9) and 75.56~127.6 μg/ml for chlorphanamine maleate (r=0.999 9).The mean recoveries of naphazoline and chlorphenamine meleate were 99.6% and 99.8%, respectively. Conclusion: The method was simple,rapid and suitable for the determination of the two constituents in cold nasal spray.

关 键 词：盐酸萘甲唑林 马来酸氯苯那敏 RP-HPLC 含量测定 

分 类 号：R972.2[医药卫生—药品]