RP-HPLC法测定归芍调经片中芍药苷的含量  

Determination of content of paeonia in Guishaotiaojing tablets by RP-HPLC

作　　者：许颖颖[1] 徐晓卫[1] 

机构地区：[1]温州医学院附属第一医院,温州325000

出　　处：《天津药学》2005年第2期21-23,共3页Tianjin Pharmacy

摘　　要：目的:应用RP-HPLC法测定归芍调经片中芍药苷的含量。方法:色谱柱为Alltech:AlltimaC18(4.6mm×150mm,5μm),流动相为乙腈-0.1%磷酸(18∶82),检测波长为230nm,流速为1.0ml/min。结果:芍药苷在0.25～2.50μg范围内进样量与峰面积呈良好线性关系(r=0.9995);平均加样回收率为98.2%,RSD=2.61%(n=5)。结论:本方法测定结果准确、灵敏、重现性好。0bject: To develop a RP-HPLC method for determination of the content of paeonia in Guishaotiaojing tablets. Methods: The separation was performed on a Alltech:Alltima C_(18) column. Acetonitrile-0.1% phosphoric acid (18∶82)was used as mobile phase, UV detection wavelength was set at 230 nm and the flow rate was 1.0 ml/min. Results: There was a good linear relationship between the concentration of paeonia and the peak areas in the range of 0.25～2.50 μg (r=0.999 5). The average recovery was 98.2% with RSD of 2.61% (n=5). Conclusion: The method was highly repeatable and accurate.

关 键 词：反相高效液相色谱法 归芍调经片 芍药苷 

分 类 号：R972.2[医药卫生—药品]