α-甘草酸对肾11β-羟基类固醇脱氢酶的抑制作用  

Inhibitory effects of α-glycyrrhizic acid on 11β-hydroxysteroid dehydrogenase

作　　者：俞进[1] 楼宜嘉[2] 

机构地区：[1]杭州市中医院,在职研究生310007 [2]浙江大学药学院,杭州310006

出　　处：《浙江医学》2005年第4期263-265,共3页Zhejiang Medical Journal

摘　　要：目的观察α-甘草酸降低底物皮质醇的转化率;探讨α-甘草酸对肾脏11β-羟基类固醇脱氢酶(11β-OHSD2)活性和血清K+浓度的影响。方法运用微粒体酶分析技术,在离体和整体实验中观察在α-甘草酸作用下,11β-OHSD2对底物皮质醇转化率的变化,并测定整体实验中豚鼠的血清K+浓度。结果(1)离体实验:在α-甘草酸作用下,11β-OHSD2对底物皮质醇的转化率为38.80%,与阴性对照(71.80%)、β-甘草酸(21.50%)相比,差别均有显著性意义(均P<0.05)。(2)整体实验:α-甘草酸中、高剂量均能明显降低豚鼠的血清K+浓度(7.08mmol?L、5.34mmol?L),与阴性对照(8.83mmol?L)、β-甘草酸(4.86mmol?L)相比,差别均有显著性意义(均P<0.05)。结论α-甘草酸能抑制豚鼠肾脏11β-OHSD2活性,导致豚鼠血清K+浓度降低,但与β-甘草酸相比,作用较弱。Objective To observe the effects ofα-glycyrrhizic acid on conversion rate of cortisol and to demonstrate the effects ofα-glycyrrhizic acid on the activity of 11β-hydroxysteroid dehydrogenase and serum K+ concentrations. Methods Using microsomes from the kidney cortex of Hartley guinea pig, the conversion rate of cortisol by α-glycyrrhizic acid was observed in vitro and in vivo, and the serum K+ concentrations were determined. Results (1) There were significantly differences in cortisol conversion rate in vitro among α-glycyrrhizic acid (38.80%), β- glycyrrhizic acid(21.50%) and the control(71.80%) (p<0.05).(2) The differences in serum K+ concentrations were also significant amongα-glycyrrhizic acid (7.08mmol/L for 150mg/Kg and 5.34mmol/L for 300 mg/Kg),β- glycyrrhizic acid(4.86mmol/L)) and the control(8.83mmol/L) (p<0.05). Conclusion α-glycyrrhizic acid can inhibit the activity of 11β-hydroxysteroid dehydrogenase and reduce K+ concentrations in serum ,but the effect is less potent than β-glycyrrhizic acid.

关 键 词：肾11β-羟基类固醇脱氢酶 Α-甘草酸 抑制作用 K^+浓度 β-甘草酸 阴性对照 mol/L 转化率 皮质醇 分析技术 微粒体酶 离体实验 显著性 底物 整体 豚鼠 相比 高剂量 鼠血清 活性 肾脏 差别 

分 类 号：R285[医药卫生—中药学]