九气拈痛胶囊对催产素所致痛经大鼠镇痛作用机制的研究  

Investigation on the Effects and Mechanism of Jiuqi Niantong Capsule (JQNTC) on Oxytocin-Induced Dysmenorrhea Rats

作　　者：彭芝配[1] 滕久祥[1] 党海珍[1] 方芳[2] 谢梦洲[1] 肖平[1] 

机构地区：[1]湖南中医学院,湖南长沙410007 [2]湖南中医学院2003级研究生班,湖南长沙410007

出　　处：《湖南中医学院学报》2005年第2期7-10,共4页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省卫生厅中医药科研基金资助项目 (2 0 0 0 77);国药准字 (Z2 0 0 10 0 0 9);国药证字 (Z2 0 0 10 0 0 6)

摘　　要：目的探讨九气拈痛胶囊对原发性痛经的镇痛作用机制。方法采用催产素所致大鼠子宫剧烈收缩造成痛经模型,拟九气拈痛胶囊3个不同剂量组,设阿司匹林、月月舒冲剂(分别为阳性组)为对照组,分别观察用药后大鼠3 0min内的扭体次数和扭体发生率,血液流变学指标,血浆中β—EP、TXB2 、6—K—PGF1α、子宫组织中PGF2α、PGE2 、NO、ET—1各项指标的变化。结果( 1 )九气拈痛胶囊3个剂量组3 0min内的扭体发生次数及其发生率分别与模型组比较具有显著性意义(P <0 .0 5 ,P <0 .0 1 ) ;( 2 )胶囊各剂量组均可改善血液流变性如全血黏度、血浆比黏度、红细胞压积、红细胞电泳时间,与模型组比较具有显著性意义(P <0 .0 5 ,P <0 .0 1 ) ;( 3 )胶囊各剂量组对大鼠血浆中β—EP、TXB2 、6—K—PGF1α含量的影响与模型组比较具有显著性意义(P <0 .0 5 ,P <0 .0 1 ) ;( 4)胶囊各组对大鼠子宫组织中NO、ET—1、PGF2α、PGE2 含量的影响分别与模型组比较具有显著性意义(P <0 .0 5 ,P <0 .0 1 )。结论九气拈痛胶囊3个剂量组对催产素所致大鼠痛经模型均具有镇痛作用,其作用与改善血液流变性、调节大鼠血浆及子宫组织中β—EP、TXB2 、PGI2 、PGE2 等分泌有关。Objective To explore the effects and the mechanism of JQNTC on primary dysmenorrhea. Methods Oxytocin induced dysmenorrhea rats were divided into modeling group, positive controlling groups (i.e. Aspirin treated group and Yueyueshu group) and three treating groups, with JQNTC given in low, medium and high dosages respectively. The number and occurrence rate of writhing (WR), the property indices of hemorheology, i.e β EP,TXB 2,6 Keto PGF 1α in plasma, and the changes of PGF 2α ,PGE 2, NO, ET 1 in uterus tissue were observed. Results Compared with those of modeling group, (1)?the number and the occurrence rate of writhing in 30 minutes in 3 dose groups of JQNTC were significantly (P<0.05) or highly significantly different (P<0.01); (2)?the property indices of hemorheology, i.e. whole blood viscosity(ηb)ηs,MCV and EFT in 3 dose groups of JQNTC were significantly(P<0.05) or highly significantly different (P<0.01); (3)?the effects on β EP,TXB 2, 6 Keto PGF 1α in plasma in 3 dose groups were significantly (P<0.05) or highly significantly different (P<0.01); (4)?the effects on the contents of NO, ET 1,PGE 2,PGF 2α in uterus tissue in 3 dose groups of JQNTC were significantly(P<0.05) or highly significantly different(P<0.01).Conclusions JQNTC can effectively relieve pain in oxyfocin induced rats.improving the property indices of hemorheology and regulating the secretinon of β EP、 TXB 2、PGI 2 and PGE 2 in plasma and uterus tissue are some of the mechanisms.

关 键 词：九气拈痛胶囊 痛经模型 催产素 作用机制 大鼠 香附 延胡索 

分 类 号：R285.5[医药卫生—中药学]