正丁醇中氯化血红素催化邻苯二胺氧化反应中间体  

Intermediate in the o-Phenylenediamine Oxidation Reaction Catalyzed by Hemin in n-Butanol

作　　者：李得加[1] 彭小伟[1] 李希文[1] 邹国林[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2005年第2期241-244,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(39770200)

摘　　要：比较了在不同有机溶剂中,以氯化血红素模拟辣根过氧化物酶催化过氧化氢氧化邻苯二胺的反应,发现在正丁醇中氯化血红素具有明显的过氧化物酶活性,能催化过氧化氢氧化邻苯二胺的反应,并可产生稳定的中间体.研究了中间体形成的动力学和不同温度、pH值和溶剂对中间体稳定性的影响.结果表明,中间体的半衰期在pH 7.5左右最大;在25 ℃时,中间体在正丁醇中的存在时间是在水中的3倍.The oxidative reaction of o-phenylenediamine with hydrogen peroxide catalyzed by hemin was detected in n-butanol. The characteristics of the reaction intermediate were studied. The experiment results show that hemin can catalyze the reaction of o-phenylenediamine with H_2O_2, and the intermediate stability of the reaction is variable as temperature and pH changed. The intermediate had the longest half life time at pH 7.5 and its exist time in n-butanol is three times as long as that in water at 25℃.

关 键 词：氯化血红素 模拟酶 中间体 正丁醇 

分 类 号：Q554.6[生物学—生物化学]