一氧化氮对大鼠肝脏缺血再灌注细胞凋亡的影响  

Effects of nitric oxide on apoptosis of liver ischemia-reperf usion damage in rats

作　　者：汪飞[1] 高晓枫[1] 林子波[1] 

机构地区：[1]惠州市中心人民医院麻醉科,广东惠州516000

出　　处：《黑龙江医药科学》2005年第2期1-2,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:探讨一氧化氮在大鼠肝脏缺血—再灌注细胞凋亡中的作用机制。方法:采用动物分组对照实验,研究了L—精氨酸(NO供体)对大鼠肝缺血再灌注细胞凋亡的影响,测量不同状态下动物肝脏组织中NO- 3/NO- 2 含量和细胞凋亡百分率(AI)的变化。结果:肝组织中一氧化氮代谢产物NO- 3/NO- 2 含量明显增加,并且肝组织中细胞凋亡百分率(AI)明显减小。结论:一氧化氮对肝脏缺血再灌注细胞凋亡有抑制作用,本实验为一氧化氮在肝缺血再灌注损伤的临床治疗提供实验基础。Objective: To observe and explore the mechanism of nitric oxide in liver i schemia reperfusion apoptosis. Methods: The effect of L-Arg on the apoptosis of liver injured by ischemia-reperfusion was studied in animal contro l experiments. The varying concentrations of NO- 3/NO- 2 and the apoptosis percentage in the liver in different conditions were detected. Results: The metabolites NO- 3/NO- 2 of nitric oxide remarkably increased, w hile the apoptosis percentage remarkably decreased in liver ischemia-reperfusion in rats (P<0.05). Conclusion: Nitric oxide has inhibitory eff ects in liver ischemia-reperfusion apoptosis, providing the experiment basis for clinical application in liver ischemia-reperfusion damage.

关 键 词：缺血再灌注 肝脏 细胞凋亡 一氧化氮 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]