人体内阿昔洛韦血药浓度的HPLC测定  

Determination of Acyclovir in human plasma by HPLC

作　　者：吴刚[1] 孙长海[1] 任淑清[1] 

机构地区：[1]佳木斯大学药学系,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2005年第2期42-43,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:建立阿昔洛韦血药浓度测定方法,用于阿昔洛韦缓释片血药浓度研究。方法:采用高效液相色谱法。分析柱为C1 8柱,流动相为2 %乙腈水溶液(p H3) ,检测波长2 5 4 nm。结果:样品组分在0 .0 5～1.0μg·ml- 1 线性良好(0 .9997) ,最低血浆药物检测浓度为0 .0 1μg·ml- 1。回收率为92 .36 %～99.2 1% ,日内及日间RSD<10 %。结论:本法简便、迅速、灵敏、准确,适合于临床药学研究需要。Objective: To develop a precise, simple and convenient method fo r the determination of Acyclovir concentration in human plasma. Methods: HPLC system was used, with C 18 as the analytical column and acetonitrile water solution (2%) as the analytical mobile phase, and 254nm as the detection wave-length. Results: The linear range of plasma Acyclovir was 0.05～1 .0μg·ml -1 (0.9997). The detection limit was 0.01μg·ml -1. The rec overy was 92.36%～99.21%, and the variation of inter-day and intra-day RSD was less than 10%. Conclusion: The method is simple, precise, sensitiv e and can be applied conveniently in studies of clinical pharmacy.

关 键 词：阿昔洛韦 血浆浓度 高效液相色谱法 

分 类 号：R927[医药卫生—药学]