水稻抗瘟性分类及品种与病菌谱系互作  

Grouping of Rice Blast Resistance and Interaction between Varieties and the Fungous Lineages

作　　者：刘二明[1] 叶华智[2] 孙雁[3] 朱有勇[3] 魏林 

机构地区：[1]湖南农业大学生物安全科技学院,湖南长沙410128 [2]四川农业大学植物保护系,四川雅安652000 [3]云南农业大学植物保护学院,云南昆明650201 [4]湖南省植物保护研究所,湖南长沙410125

出　　处：《湖南农业大学学报（自然科学版）》2005年第2期147-152,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家"863"计划资助项目(Z16-03-M)

摘　　要：选用两对引物XLRRfor/XLRRrev和S1/AS3对23个水稻品种进行RGA-PCR指纹聚类分析,以80%的相似性可将23个品种分成9类;进一步对这23个品种进行抗瘟性表型聚类分析.结果表明,虽然两种方法划分的品种组别数相同,有2个组别的品种组成也相同,但多数组别的品种组成有差异,即存在R-基因与抗性表型不一致的现象.因此,RGA-PCR指纹分析法与传统的抗性表型鉴定法相结合才能获得准确、可靠的抗性信息.品种RGA和抗性表型相似组别与病菌谱系之间没有明确的对应关系,但就单个品种而言,某些品种与一些病原菌谱系出现即皆亲和或皆非亲和的互作现象.Two primer pairs , viz . XLRRfor/XLRRrev and S1/AS3 , were used to fingerprint 23 rice varieties.Cluster analysis showed that 23 varieties could be attributed in 9 groups at 80% similarity level.Further cluster analysis was also made for the same sets of varieties based on phenotypic traits,which were associated with blast resistance . The comparison of the two clustering results suggested the same number of variety groups.However,variety composition in each group (except two groups separately with one variety) was different,indicating the differences between R-genes and resistant phenotype.However there was all compatible or incompatible interaction in some varieties and lineage.

关 键 词：稻瘟病菌 抗性基因同源序列 抗性表型 谱系 互作 

分 类 号：S431.11[农业科学—农业昆虫与害虫防治]